Anti-LMAN1抗体(ab32583)
Key features and details
- Rabbit polyclonal to LMAN1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LMAN1抗体
参阅全部 LMAN1 一抗 -
描述
兔多克隆抗体to LMAN1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide:
FDSFDNDGKKNNPAI
, corresponding to amino acids 159-173 of Rat LMAN1 -
阳性对照
- ICC: p19 cells, HMVEC cells, A549 cells. WB: Rat brain lysate. HepG2, HeLa, PANC-1, and MCF7 lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32583于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 57.5 kDa.
|
说明 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 57.5 kDa. |
靶标
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功能
Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. -
组织特异性
Ubiquitous. -
疾病相关
Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1) [MIM:227300]; also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal. -
序列相似性
Contains 1 L-type lectin-like domain. -
翻译后修饰
The N-terminal may be partly blocked. -
细胞定位
Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3998 Human
- Entrez Gene: 70361 Mouse
- Entrez Gene: 116666 Rat
- Omim: 601567 Human
- SwissProt: P49257 Human
- SwissProt: Q9D0F3 Mouse
- SwissProt: Q62902 Rat
- Unigene: 465295 Human
see all -
别名
- Endoplasmic reticulum golgi intermediate compartment protein 53 antibody
- ER-Golgi intermediate compartment 53 kDa protein antibody
- ERGIC-53 antibody
see all
图片
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ab32583 labelling LMAN1 in p19 cells by Immunocytochemistry/Immunofluorescence.
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ab32583 labelling LMAN1 in HMVEC cells by Immunocytochemistry/Immunofluorescence.
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All lanes : Anti-LMAN1 antibody (ab32583) at 1/2000 dilution
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : PANC-1 lysate
Lane 4 : MCF7 lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution -
ab32583 labelling LMAN1 in A549 cells by Immunocytochemistry/Immunofluorescence.
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All lanes : Anti-LMAN1 antibody (ab32583) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate with Immunising peptide
Observed band size: 57.5 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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Datasheet download
文献 (0)
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