概述

  • 产品名称Anti-LLGL1抗体
    参阅全部 LLGL1 一抗
  • 描述
    兔多克隆抗体to LLGL1
  • 经测试应用适用于: WBmore details
  • 种属反应性

    预测可用于: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Lgl1.

    (Peptide available as ab39291.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates; Human brain and Mouse brain.

性能

应用

Our Abpromise guarantee covers the use of ab39292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).

靶标

  • 功能Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells.
  • 组织特异性Expressed in brain, kidney, and muscle but is barely seen in heart and placenta. Down-regulated or lost in all cell lines and in most of the tumor samples analyzed. Loss was associated with advanced stage of the disease.
  • 序列相似性Belongs to the WD repeat L(2)GL family.
    Contains 14 WD repeats.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated at least at Ser-663 by PRKCI.
  • 细胞定位Cytoplasm > cytoskeleton. Localized to the lateral membrane during the polarization and formation cell-cell contacts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DLG4 antibody
    • DLG4, formerly antibody
    • Hugl 1 antibody
    • HUGL antibody
    • Hugl-1 antibody
    • HUGL1 antibody
    • Human homolog to the D-lgl gene protein antibody
    • L2GL1_HUMAN antibody
    • Lethal giant larvae homolog 1 (Drosophila) antibody
    • Lethal giant larvae homolog 1 antibody
    • Lethal(2) giant larvae protein homolog 1 antibody
    • LLGL antibody
    • Llgl1 antibody
    • Ly 49G2 antibody
    • Ly49G2 antibody
    • Mgl1 antibody
    • Mlgl antibody
    see all

Anti-LLGL1 antibody 图像

  • All lanes : Anti-LLGL1 antibody (ab39292) at 1 µg

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 115 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa,30 kDa,50 kDa. We are unsure as to the identity of these extra bands.

Anti-LLGL1 antibody (ab39292)参考文献

This product has been referenced in:
  • Song J  et al. Hugl-1 induces apoptosis in esophageal carcinoma cells both in vitro and in vivo. World J Gastroenterol 19:4127-36 (2013). WB ; Human . Read more (PubMed: 23864775) »

See 1 Publication for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (various: incl. SW620(+) HEK293 Ms Brain)
Loading amount 30 µg
Specification various: incl. SW620(+) HEK293 Ms Brain
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Nov 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"