The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).
功能Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells.
组织特异性Expressed in brain, kidney, and muscle but is barely seen in heart and placenta. Down-regulated or lost in all cell lines and in most of the tumor samples analyzed. Loss was associated with advanced stage of the disease.
序列相似性Belongs to the WD repeat L(2)GL family. Contains 14 WD repeats.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated at least at Ser-663 by PRKCI.
细胞定位Cytoplasm > cytoskeleton. Localized to the lateral membrane during the polarization and formation cell-cell contacts.