The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125-0.25µg for 105-8 cells. Perform staining in a final volume of 100 µl.
ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
功能Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells.
组织特异性Expressed in brain, kidney, and muscle but is barely seen in heart and placenta. Down-regulated or lost in all cell lines and in most of the tumor samples analyzed. Loss was associated with advanced stage of the disease.
序列相似性Belongs to the WD repeat L(2)GL family. Contains 14 WD repeats.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated at least at Ser-663 by PRKCI.
细胞定位Cytoplasm > cytoskeleton. Localized to the lateral membrane during the polarization and formation cell-cell contacts.
Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha2) PE and 0.125 µg of Rat IgG2a k Isotype Control FITC (left) or 0.125 µg of ab95802 (right). Total viable cells were used for analysis.
Anti-LLGL1 antibody [4D11] (FITC) (ab95802)参考文献
This product has been referenced in:
Ortaldo JR et al. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J Immunol160:1158-65 (1998).
Read more (PubMed: 9570529) »
Mason LH et al. Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2) J Exp Med182:293-303 (1995).
Read more (PubMed: 7629495) »