Anti-LKB1 (phospho S428)抗体(ab63473)

概述

  • 产品名称
    Anti-LKB1 (phospho S428)抗体
    参阅全部 LKB1 一抗
  • 描述
    兔多克隆抗体to LKB1 (phospho S428)
  • 特异性
    ab63473 detects endogenous levels of LKB1 only when phosphorylated at serine 428.
  • 经测试应用
    适用于: ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of serine 428 (R-L-SP-A-C).

  • 阳性对照
    • WB: Mouse brain extracts. ICC/IF: NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab63473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.

靶标

  • 功能
    Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
  • 组织特异性
    Ubiquitously expressed. Strongest expression in testis and fetal liver.
  • 疾病相关
    Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
    Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylated by a cAMP-dependent protein kinase.
  • 细胞定位
    Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hLKB1 antibody
    • Liver kinase B1 antibody
    • LKB1 antibody
    • PJS antibody
    • Polarization related protein LKB1 antibody
    • Renal carcinoma antigen NY-REN-19 antibody
    • Serine/Threonine Kinase 11 antibody
    • Serine/threonine protein kinase 11 antibody
    • Serine/threonine protein kinase LKB1 antibody
    • Serine/threonine protein kinase STK11 antibody
    • Serine/threonine-protein kinase 11 antibody
    • Serine/threonine-protein kinase LKB1 antibody
    • Serine/threonine-protein kinase XEEK1 antibody
    • Stk11 antibody
    • STK11_HUMAN antibody
    see all

图片

  • All lanes : Anti-LKB1 (phospho S428) antibody (ab63473) at 1/500 dilution

    Lane 1 : mouse brain extracts
    Lane 2 : mouse brain extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • ab63473 at 1/500 dilution staining LKB1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

文献

This product has been referenced in:
  • Sueda T  et al. BRAF V600E inhibition stimulates AMP-activated protein kinase-mediated autophagy in colorectal cancer cells. Sci Rep 6:18949 (2016). Read more (PubMed: 26750638) »
  • Yang SH  et al. Lovastatin overcomes gefitinib resistance through TNF-a signaling in human cholangiocarcinomas with different LKB1 statuses in vitro and in vivo. Oncotarget 6:23857-73 (2015). WB, IHC . Read more (PubMed: 26160843) »

See all 3 Publications for this product

客户评价及客户问答

Thank you for contacting us.

The link to the database entry for the human LKB1 protein is:

http://www.uniprot.org/uniprot/Q15831

I hope this information is helpful to you. Please do not hesitate to contact us if you need...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain)
Loading amount
50 µg
Specification
Mouse brain
Gel Running Conditions
Reduced Denaturing (10-20% tris-tricine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Anna Lauer

Verified customer

提交于 May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册