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Highly purified recombinant full length protein made in E. coli..
Our Abpromise guarantee covers the use of ab15095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 48.6 kDa).|
Negative control (Lane 1): LKB1 knockout MEF cells.
Positive control (Lane 2): wildtype MEF cells.
Blocking step: 5% milk as blocking agent for 1 hour at 25 °C.
Incubation: 16 hours at 4 °C, using 5% milk in TBS/0.1 Tween as diluent.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: LKB1 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab15095 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab15095 was shown to recognize LKB1 when LKB1 knockout samples were used, along with additional cross-reactive bands. Wild-type and LKB1 knockout samples were subjected to SDS-PAGE. ab15095 and ab181602 (loading control to LKB1) were diluted 1/100 and 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
ab15095 staining LKB1 in human hepatocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Citrate buffer ph 6 for 20 minutes at 97ºC. Samples were incubated with primary antibody (1/100 in diluent) for 1 hour at 37°C. A HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
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