Anti-LKB1抗体- C-terminal (ab185734)
Key features and details
- Rabbit polyclonal to LKB1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LKB1抗体- C-terminal
参阅全部 LKB1 一抗 -
描述
兔多克隆抗体to LKB1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human LKB1 aa 300 to the C-terminus (C terminal).
Database link: Q15831 -
阳性对照
- MCF-7 and HepG2 extract
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab185734于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 49 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 49 kDa. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor. -
组织特异性
Ubiquitously expressed. Strongest expression in testis and fetal liver. -
疾病相关
Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated by a cAMP-dependent protein kinase. -
细胞定位
Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2. - Information by UniProt
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数据库链接
- Entrez Gene: 6794 Human
- Entrez Gene: 20869 Mouse
- Entrez Gene: 314621 Rat
- Omim: 602216 Human
- SwissProt: Q15831 Human
- SwissProt: Q9WTK7 Mouse
- SwissProt: D4AE59 Rat
- Unigene: 515005 Human
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别名
- hLKB1 antibody
- Liver kinase B1 antibody
- LKB1 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab185734 被引用在 5 文献中.
- Puustinen P et al. DNA-dependent protein kinase regulates lysosomal AMP-dependent protein kinase activation and autophagy. Autophagy 16:1871-1888 (2020). PubMed: 31983282
- Ideno N et al. A pipeline for rapidly generating genetically engineered mouse models of pancreatic cancer using in vivo CRISPR-Cas9-mediated somatic recombination. Lab Invest N/A:N/A (2019). PubMed: 30728464
- Kari S et al. PKA Activates AMPK Through LKB1 Signaling in Follicular Thyroid Cancer. Front Endocrinol (Lausanne) 10:769 (2019). PubMed: 31798532
- Zhang W et al. LKB1 loss cooperating with BRAF V600E promotes melanoma cell invasion and migration by up-regulation MMP-2 via PI3K/Akt/mTOR pathway. Oncotarget 8:113847-113857 (2017). PubMed: 29371951
- Presneau N et al. Post-translational regulation contributes to the loss of LKB1 expression through SIRT1 deacetylase in osteosarcomas. Br J Cancer 117:398-408 (2017). PubMed: 28632727