概述

  • 产品名称
  • 描述
    兔多克隆抗体to LIX1L
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 159-189 of Human LIX1L (NP_714924.1).

  • 阳性对照
    • MDA-MB231 cell line and Mouse heart tissue lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab112952 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab112952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 37 kDa.

靶标

图片

  • ab112952 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab112952 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-LIX1L antibody (ab112952) at 1/100 dilution + MDA-MB231 cell line lysate at 35 µg

    Predicted band size : 37 kDa
  • Anti-LIX1L antibody (ab112952) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 37 kDa

文献

ab112952 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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