Anti-liver FABP抗体[L2B10] (ab7366)

概述

  • 产品名称
    Anti-liver FABP抗体[L2B10]
    参阅全部 liver FABP 一抗
  • 描述
    小鼠单克隆抗体[L2B10] to liver FABP
  • 特异性
    This monoclonal antibody binds to human liver FABP of both natural and recombinant origin. No reactivity to human intestinal or heart FABP.
  • 经测试应用
    适用于: Flow Cyt, Functional Studies, IHC-P, WB, IHC-Frmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, African green monkey

性能

应用

Our Abpromise guarantee covers the use of ab7366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Functional Studies Use at an assay dependent concentration. Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37°C, 5% CO2. * T=24: measure L-FABP in supernatant.
IHC-P Use at an assay dependent concentration.
WB 1/500. PubMed: 18779054
IHC-Fr Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能
      Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
    • 序列相似性
      Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
    • 结构域
      Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FABP 1 antibody
      • FABP1 antibody
      • FABPL antibody
      • FABPL_HUMAN antibody
      • Fatty Acid Binding Protein 1 antibody
      • Fatty acid binding protein 1 liver antibody
      • Fatty Acid Binding Protein antibody
      • Fatty acid-binding protein 1 antibody
      • Fatty acid-binding protein antibody
      • Fatty acid-binding protein liver antibody
      • L FABP antibody
      • L-FABP antibody
      • liver antibody
      • Liver-type fatty acid-binding protein antibody
      see all

    Anti-liver FABP antibody [L2B10] 图像

    • Immunohistochemical analysis of human L-FABP in frozen human kidney tissue with ab7366 at 1.9 μg/ml.

    • Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 200X.

    • Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 50X.

    • Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-liver FABP antibody [L2B10] (ab7366)参考文献

    This product has been referenced in:
    • Takahashi T  et al. Histopathological characteristics of glutamine synthetase-positive hepatic tumor lesions in a mouse model of spontaneous metabolic syndrome (TSOD mouse). Mol Clin Oncol 5:267-270 (2016). IHC-P ; Mouse . Read more (PubMed: 27446562) »
    • Smathers RL  et al. Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver. J Lipid Res 54:1335-45 (2013). WB ; Mouse . Read more (PubMed: 23359610) »

    See all 8 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Cow Tissue lysate - whole (liver tissue lysate)
    Loading amount
    10 µg
    Specification
    liver tissue lysate
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 10 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African Green Monkey Cell (hepatocyte)
    Specification
    hepatocyte
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 29 2008

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (hepatocyte)
    Specification
    hepatocyte
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 29 2008

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (hepatocytes)
    Specification
    hepatocytes
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% triton x-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 20 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (cos-7)
    Loading amount
    75 µg
    Specification
    cos-7
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Blocking step
    Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 18 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (hepatocytes)
    Loading amount
    50 µg
    Specification
    hepatocytes
    Gel Running Conditions
    Reduced Non-Denaturing (Native)
    Blocking step
    Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 18 2008

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (primary hepatocytes)
    Specification
    primary hepatocytes
    Fixative
    Methanol
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    提交于 Nov 21 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (liver)
    Loading amount
    25 µg
    Specification
    liver
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 07 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary Hepatocytes)
    Loading amount
    25 µg
    Specification
    Primary Hepatocytes
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 25 2007

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    50 µg
    Specification
    liver
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Dr. Ying Chen

    Verified customer

    提交于 Oct 12 2007

    1-10 of 11 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册