Anti-liver FABP抗体[2G4] (ab82157)

概述

  • 产品名称Anti-liver FABP抗体[2G4]
    参阅全部 liver FABP 一抗
  • 描述
    小鼠单克隆抗体[2G4] to liver FABP
  • 经测试应用适用于: Flow Cyt, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human liver FABP (aa1-127) purified from E. coli (NP_001434)

  • 阳性对照
    • HepG2 cell lysates and Human colon cancer
  • 常规说明Isotype: Mouse IgG1 heavy chain and kappa light chain

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号2G4
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/100 - 1/300. for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer.
WB 1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ELISA Use at an assay dependent concentration.

靶标

  • 功能Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
  • 序列相似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • 结构域Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FABP 1 antibody
    • FABP1 antibody
    • FABPL antibody
    • FABPL_HUMAN antibody
    • Fatty Acid Binding Protein 1 antibody
    • Fatty acid binding protein 1 liver antibody
    • Fatty Acid Binding Protein antibody
    • Fatty acid-binding protein 1 antibody
    • Fatty acid-binding protein antibody
    • Fatty acid-binding protein liver antibody
    • L FABP antibody
    • L-FABP antibody
    • liver antibody
    • Liver-type fatty acid-binding protein antibody
    see all

Anti-liver FABP antibody [2G4] 图像

  • Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution + HepG2 cell lysate at 30 µg

    Secondary
    goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • ab82157 at 1/100 dilution staining liver FABP in human colon cancer by Immunohistochemistry, Paraffin-embedded tissue detected using Diaminobenzidine (DAB).
  • Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-liver FABP antibody [2G4] (ab82157)参考文献

ab82157 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"