Anti-liver FABP抗体[2G4] (ab82157)


  • 产品名称Anti-liver FABP抗体[2G4]
    参阅全部 liver FABP 一抗
  • 描述
    小鼠单克隆抗体[2G4] to liver FABP
  • 经测试应用适用于: Flow Cyt, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human liver FABP (aa1-127) purified from E. coli (NP_001434)

  • 阳性对照
    • HepG2 cell lysates and Human colon cancer
  • 常规说明Isotype: Mouse IgG1 heavy chain and kappa light chain


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号2G4
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab82157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/100 - 1/300. for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer.
WB 1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ELISA Use at an assay dependent concentration.


  • 功能Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
  • 序列相似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • 结构域Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FABP 1 antibody
    • FABP1 antibody
    • FABPL antibody
    • FABPL_HUMAN antibody
    • Fatty Acid Binding Protein 1 antibody
    • Fatty acid binding protein 1 liver antibody
    • Fatty Acid Binding Protein antibody
    • Fatty acid-binding protein 1 antibody
    • Fatty acid-binding protein antibody
    • Fatty acid-binding protein liver antibody
    • L FABP antibody
    • L-FABP antibody
    • liver antibody
    • Liver-type fatty acid-binding protein antibody
    see all

Anti-liver FABP antibody [2G4] 图像

  • Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution + HepG2 cell lysate at 30 µg

    goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • ab82157 at 1/100 dilution staining liver FABP in human colon cancer by Immunohistochemistry, Paraffin-embedded tissue detected using Diaminobenzidine (DAB).
  • Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-liver FABP antibody [2G4] (ab82157)参考文献

ab82157 has not yet been referenced specifically in any publications.

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Unfortunately, we offer the antibody only in the size listed on the datasheet (100 ul). Sample sizes cannot be offered due to operational reasons. For larger sizes or bulk orders, please contact mailto:sales@abca...

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