概述
-
产品名称Anti-Liver Carboxylesterase 1抗体[EP1376Y]
参阅全部 Liver Carboxylesterase 1 一抗 -
描述兔单克隆抗体[EP1376Y] to Liver Carboxylesterase 1
-
宿主Rabbit
-
经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性与反应: Human
-
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Liver Carboxylesterase 1 aa 550 to the C-terminus (C terminal).
-
阳性对照
- U937 cell lysates Human liver tissue
-
常规说明
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
-
纯度Tissue culture supernatant
-
克隆单克隆
-
克隆编号EP1376Y
-
同种型IgG
-
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
应用
Our Abpromise guarantee covers the use of ab53008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa). | |
| IP | 1/100. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/50 - 1/100. |
靶标
-
功能Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
-
组织特异性Expressed predominantly in liver with lower levels in heart and lung.
-
序列相似性Belongs to the type-B carboxylesterase/lipase family.
-
翻译后修饰Contains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. -
细胞定位Endoplasmic reticulum lumen.
- Information by UniProt
-
数据库链接
- Entrez Gene: 1066 Human
- Omim: 114835 Human
- SwissProt: P23141 Human
- Unigene: 558865 Human
-
别名
- ACAT antibody
- Acyl coenzyme A cholesterol acyltransferase antibody
- Acyl-coenzyme A:cholesterol acyltransferase antibody
see all
图片
-
![Western blot - Anti-Liver Carboxylesterase 1 antibody [EP1376Y] (ab53008)](http://www.abcam.cn/ps/products/53/ab53008/Images/ab53008_WB_1.jpg)
Western blot - Anti-Liver Carboxylesterase 1 antibody [EP1376Y] (ab53008)Anti-Liver Carboxylesterase 1 antibody [EP1376Y] (ab53008) at 1/10000 dilution + U937 cell lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Carboxylesterase 1 antibody [EP1376Y] (ab53008)](http://www.abcam.cn/ps/products/53/ab53008/Images/ab53008_IHC_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Carboxylesterase 1 antibody [EP1376Y] (ab53008)ab53008, at a 1/250 dilution, staining Human SOAT 1 in Liver using Immunohistochemistry, Paraffin embedded tissue.
文献
ab53008 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"