The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/25000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 62 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC/IF or IHC-P.
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
Expressed predominantly in liver with lower levels in heart and lung.
Belongs to the type-B carboxylesterase/lipase family.
Contains sialic acid. Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.
Ab68190 staining Liver Carboxylesterase 1 in U-937 (Human histiocytic lymphoma monocyte) by Flow Cytometry. Cells were fixed with 2% paraformaldehyde. The sample was incubated with primary antibody at 1:150 dilution (pink), A Goat anti-rabbit IgG (FITC) was used as a secondary antibody at 1:150 dilution. Rabbit monoclonal IgG was used as the isotype control (green).