重组Anti-Liver Carboxylesterase 1/CES1抗体[EP1375Y] (ab68190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1375Y] to Liver Carboxylesterase 1/CES1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Liver Carboxylesterase 1/CES1抗体[EP1375Y]
参阅全部 Liver Carboxylesterase 1/CES1 一抗 -
描述
兔单克隆抗体[EP1375Y] to Liver Carboxylesterase 1/CES1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Liver Carboxylesterase 1/CES1 (C terminal). The exact sequence is proprietary.
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阳性对照
- Flow Cyt (intra): U-937 cells; WB: U-937 cells, mouse and rat kidney lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.5% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1375Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68190于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/25000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 62 kDa).
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说明 |
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Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/25000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 62 kDa). |
靶标
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功能
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. -
组织特异性
Expressed predominantly in liver with lower levels in heart and lung. -
序列相似性
Belongs to the type-B carboxylesterase/lipase family. -
翻译后修饰
Contains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
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数据库链接
- Entrez Gene: 1066 Human
- Entrez Gene: 12623 Mouse
- Entrez Gene: 24346 Rat
- Entrez Gene: 29225 Rat
- Omim: 114835 Human
- SwissProt: P23141 Human
- SwissProt: Q8VCC2 Mouse
- SwissProt: P10959 Rat
see all -
别名
- ACAT antibody
- Acyl coenzyme A cholesterol acyltransferase antibody
- Acyl-coenzyme A:cholesterol acyltransferase antibody
see all
图片
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All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190) at 1/50000 dilution (Purified)
Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 21049984 and PMID: 30513753).
Blocking Buffer and concentration: 5% NFDM/TBST
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Intracellular Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling Liver Carboxylesterase 1/CES1 with Purified ab68190 at 1/70 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab68190 被引用在 5 文献中.
- Jiang LP et al. Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice. Br J Pharmacol 179:46-64 (2022). PubMed: 34415054
- Qin M et al. Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis. Cells 11:N/A (2022). PubMed: 35053380
- Wu Y et al. Effects of ABCG1 knockout on proteomic composition of HDL in mice on a chow diet and a high-fat diet. Proteomics 22:e2100028 (2022). PubMed: 35234362
- Ross NT et al. CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma. Nat Chem Biol 16:50-59 (2020). PubMed: 31819276
- Bai J et al. Sodium sulfite promotes the assembly and secretion of very low-density lipoprotein in HL-7702 hepatocytes. Toxicol Rep 3:98-104 (2016). PubMed: 28959527