Anti-Liver Carboxylesterase 1/CES1抗体(ab45957)
Key features and details
- Rabbit polyclonal to Liver Carboxylesterase 1/CES1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Liver Carboxylesterase 1/CES1抗体
参阅全部 Liver Carboxylesterase 1/CES1 一抗 -
描述
兔多克隆抗体to Liver Carboxylesterase 1/CES1 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Baboon, Rhesus monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45957于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. -
组织特异性
Expressed predominantly in liver with lower levels in heart and lung. -
序列相似性
Belongs to the type-B carboxylesterase/lipase family. -
翻译后修饰
Contains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
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数据库链接
- Entrez Gene: 1066 Human
- Entrez Gene: 12623 Mouse
- Entrez Gene: 24346 Rat
- Entrez Gene: 29225 Rat
- Omim: 114835 Human
- SwissProt: P23141 Human
- SwissProt: Q8VCC2 Mouse
- SwissProt: P10959 Rat
see all -
别名
- ACAT antibody
- Acyl coenzyme A cholesterol acyltransferase antibody
- Acyl-coenzyme A:cholesterol acyltransferase antibody
see all
图片
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All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody (ab45957) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole cell lysate
Lane 2 : THP-1 whole cell lysate (ab7913)
Lane 3 : Liver (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 63 kDa -
ICC/IF image of ab45957 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45957, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% Methanol fixed (5 min) HepG2 cells at 1µg/ml.
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IHC image of Carboxylesterase 1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45957, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab45957 被引用在 10 文献中.
- Shi J et al. Plasma Carboxylesterase 1 Predicts Methylphenidate Exposure: A Proof-of-Concept Study Using Plasma Protein Biomarker for Hepatic Drug Metabolism. Clin Pharmacol Ther 111:878-885 (2022). PubMed: 34743324
- Xu Y et al. Hepatocyte-Specific Expression of Human Carboxylesterase 1 Attenuates Diet-Induced Steatohepatitis and Hyperlipidemia in Mice. Hepatol Commun 4:527-539 (2020). PubMed: 32258948
- Wen X et al. Hepatic carboxylesterases are differentially regulated in PPARa-null mice treated with perfluorooctanoic acid. Toxicology 416:15-22 (2019). PubMed: 30685356
- Xu Y et al. Lipocalin-2 Protects Against Diet-Induced Nonalcoholic Fatty Liver Disease by Targeting Hepatocytes. Hepatol Commun 3:763-775 (2019). PubMed: 31168511
- Xu J et al. Global inactivation of carboxylesterase 1 (Ces1/Ces1g) protects against atherosclerosis in Ldlr-/-mice. Sci Rep 7:17845 (2017). PubMed: 29259301
- Li J et al. Sortilin 1 Modulates Hepatic Cholesterol Lipotoxicity in Mice via Functional Interaction with Liver Carboxylesterase 1. J Biol Chem 292:146-160 (2017). PubMed: 27881673
- Fu J et al. Biotransformation Capacity of Carboxylesterase in Skin and Keratinocytes for the Penta-Ethyl Ester Prodrug of DTPA. Drug Metab Dispos 44:1313-8 (2016). PubMed: 27130352
- Xu J et al. Carboxylesterase 1 Is Regulated by Hepatocyte Nuclear Factor 4a and Protects Against Alcohol- and MCD diet-induced Liver Injury. Sci Rep 6:24277 (2016). WB . PubMed: 27075303
- Miwa S et al. Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H2O2 release assays. Free Radic Biol Med 90:173-83 (2016). PubMed: 26577176
- Xu J et al. Hepatic carboxylesterase 1 is induced by glucose and regulates postprandial glucose levels. PLoS One 9:e109663 (2014). WB ; Mouse . PubMed: 25285996