Anti-Liver Arginase抗体(ab92274)

概述

  • 产品名称
    Anti-Liver Arginase抗体
    参阅全部 Liver Arginase 一抗
  • 描述
    山羊多克隆抗体to Liver Arginase
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
    预测可用于: Rabbit, Cow, Dog
  • 免疫原

    Synthetic peptide:

    CFGLAREGNHKPID

    , corresponding to C terminal amino acids 303-316 of Human liver Arginase (NP_000036.2).

  • 阳性对照
    • Human liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab92274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.

靶标

  • 通路
    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • 疾病相关
    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • 序列相似性
    Belongs to the arginase family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

图片

  • Immunohistochemistry (paraffin-embedded sections) analysis of human kidney tissue labeling Liver Arginase with ab92274 at 4µg/ml. Microwaved antigen retrieval was performed using Tris/EDTA buffer pH9. HRP-staining.

  • All lanes : Anti-Liver Arginase antibody (ab92274) at 0.03 µg/ml

    Lane 1 : pig liver tissue lysate
    Lane 2 : mouse liver tissue lysate
    Lane 3 : rat liver tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Anti-Liver Arginase antibody (ab92274) at 0.01 µg/ml + human liver lysate in RIPA buffer at 35 µg

    Predicted band size : 35 kDa

文献

ab92274 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Pig Tissue lysate - whole (myocardium)
Loading amount
50 µg
Specification
myocardium
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Rahul Dalal

Verified customer

提交于 Sep 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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