Anti-liver Arginase抗体(ab91279)

概述

  • 产品名称
    Anti-liver Arginase抗体
    参阅全部 Liver Arginase 一抗
  • 描述
    兔多克隆抗体to liver Arginase
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Pig
  • 免疫原

    Synthetic peptide corresponding to Mouse Liver Arginase aa 300 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab100829, ab100829)

  • 阳性对照
    • This antibody gave a positive signal in mouse and rat liver tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab91279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.

靶标

  • 通路
    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • 疾病相关
    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • 序列相似性
    Belongs to the arginase family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

Anti-liver Arginase antibody 图像

  • All lanes : Anti-liver Arginase antibody (ab91279) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 37,40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Liver Arginase was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Liver Arginase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab91279.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 37, 40kDa: liver Arginase
  • ICC/IF image of ab91279 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91279, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-liver Arginase antibody (ab91279)参考文献

This product has been referenced in:
  • Ballantyne LL  et al. Liver-specific knockout of arginase-1 leads to a profound phenotype similar to inducible whole body arginase-1 deficiency. Mol Genet Metab Rep 9:54-60 (2016). IHC-P, IF, WB ; Mouse . Read more (PubMed: 27761413) »
  • Yang J  et al. Interleukin-4 Ameliorates the Functional Recovery of Intracerebral Hemorrhage Through the Alternative Activation of Microglia/Macrophage. Front Neurosci 10:61 (2016). WB, IHC-FoFr ; Rat . Read more (PubMed: 27013935) »

See all 4 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Permeabilization
Yes - 0.2% Tween 20
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Stuart Young

Verified customer

提交于 Feb 28 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
10% donkey serum 3% BSA 0.3% triton-x100 as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 18 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 14 2014

DISCOUNT CODE: ***
Expiration date: September 1st, 2012

I am very pleased to hear you would like to accept our offer and test ab64693 in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this off...

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The current lot of ab91279, and any lots of at least 1mg/ml, will not contain BSA but will contain 0.02% Sodium azide. I hope this is helpful. Please contact me again if you need any further information.

Sorry for the late reply. I am still enquirying as to the availability of these 2 antibodies in BSA-free and azide-free formulations. Thanks for your patience!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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