Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver)
Specification
liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: diluent from another company
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/1000
Additional data
Additional Notes
staining of nuclei instead of cytoplasm
Abcam response
Thank you for submitting your Abreview. While the immunogen for ab96183 shows high homology with mouse, we have not validated this antibody for use on mouse samples. Based on your feedback it may not be suitable for use on that species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. R Berahovich
Verified customer
提交于 May 17 2012