概述

  • 产品名称Anti-LITAF抗体
    参阅全部 LITAF 一抗
  • 描述
    小鼠多克隆抗体to LITAF
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human protein (NP_004853.2)

  • 阳性对照
    • WB: placenta lysate; IHC: prostate tissue; ICC/IF: HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab88742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Microwave method recommended.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF-alpha) gene expression.
    • 组织特异性Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen.
    • 疾病相关Defects in LITAF are the cause of Charcot-Marie-Tooth disease type 1C (CMT1C) [MIM:601098]. CMT1C is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet.
      Note=Defects in LITAF may be involved in extramammary Paget disease (EMPD) carcinogenesis. EMPD is a cancerous disease representing about 8% of all malignant skin cancers; it usually appears in the anogenital area and can be fatal by metastasizing to internal organs when left untreated for a long time. The clinical features are usually those of eczematous eruptions with weeping and crust formation.
    • 结构域The WW-binding motif mediates interaction with WWOX and, probably NEDD4.
    • 细胞定位Lysosome membrane. Associated with membranes of lysosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Lipopolysaccharide induced TNF alpha factor antibody
      • CMT1C antibody
      • FLJ38636 antibody
      • Lipopolysaccharide induced TNF alpha factor antibody
      • Lipopolysaccharide induced TNF factor antibody
      • Lipopolysaccharide induced tumor necrosis factor alpha factor antibody
      • Lipopolysaccharide-induced tumor necrosis factor-alpha factor antibody
      • LITAF antibody
      • LITAF_HUMAN antibody
      • LPS induced TNF alpha factor antibody
      • LPS-induced TNF-alpha factor antibody
      • MGC116698 antibody
      • MGC116700 antibody
      • MGC116701 antibody
      • MGC125274 antibody
      • MGC125275 antibody
      • MGC125276 antibody
      • p53 induced gene 7 protein antibody
      • p53-induced gene 7 protein antibody
      • PIG 7 antibody
      • PIG7 antibody
      • SIMPLE antibody
      • Small integral membrane protein of lysosome/late endosome antibody
      • TP53I7 antibody
      • Tumor protein p53 inducible protein 7 antibody
      see all

    Anti-LITAF antibody 图像

    • Anti-LITAF antibody (ab88742) at 1 µg/ml + Human placenta lysate at 50 µg

      Predicted band size : 17 kDa
    • All lanes : Anti-LITAF antibody (ab88742) at 1 µg/ml

      Lane 1 : LITAF transfected 293T cell lysate
      Lane 2 : Untransfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 17 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • Immunoperoxidase staining of formalin-fixed, paraffin-embedded Human prostate tissue using ab88742 at 3 µg/ml.
    • Immunofluorescence analysis of HeLa cells using ab88742 at 10 µg/ml followed by a fluorescein-conjugated secondary antibody.

    Anti-LITAF antibody (ab88742)参考文献

    ab88742 has not yet been referenced specifically in any publications.

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