Anti-Listeria monocytogenes抗体[LZA2] (ab11438)

概述

  • 产品名称Anti-Listeria monocytogenes抗体[LZA2]
    参阅全部 Listeria monocytogenes 一抗
  • 描述
    小鼠单克隆抗体[LZA2] to Listeria monocytogenes
  • 特异性Recognises L. Monocytogenes outer membrane fraction and whole cells.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    This antibody does not react with the following species: E. coli, Salmonella, Y. pestis, F. tularensis, L. pneumophila and B. abortus.
  • 免疫原

    Listeria monocytogenes.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Purified IgM
  • 纯化说明Clone LZA2 is purified by euglobulin precipitation. Purity tested by electrophoresis.
  • 克隆单克隆
  • 克隆编号LZA2
  • 骨髓瘤Sp2/0
  • 同种型IgM
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明Direct ELISA: Use at a concentration of 1ng/ml - 3 µg/ml.
    Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at + 4 °C. Add ab11438 in PBST, 1 h, 37 °C.
    Use anti-mouse IgG HRP-conjugated in PBST 1 hr, 37 °C. Substrate: TMB, 30 min.

    WB: Use at a concentration of 8 µg/ml. Use 15 % SDS-PAGE electrophoresis in reducing conditions (30 ?g antigen per track) and transfer to nitrocellulose membrane (60 min, 100 mA). Blocking: 1 % BSA in PBS, 1 h, 37 °C.
    Detects a band of approximately 23 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
    • 别名
      • L. monocytogenes antibody

    Anti-Listeria monocytogenes antibody [LZA2] (ab11438)参考文献

    ab11438 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

    Thank you for your enquiry. The concentration of this lot of the antibody is 4.40 mg/ml. The size we sell is 100ug, but it appears that you actually received nearly double that amount! I hope this information helps. Please do not hesitate to c...

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    I enclose the protocols that were used to test these antibodies: Direct ELISA: Use at a concentration of 1ng/ml - 3 µg/ml. Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at +4°C. Add ab...

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    Thank you for your enquiry regarding our Salmonella typhimurium, Listeria monocytogenes, or Campylobacter jejuni antibodies. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (ab11440)(capture – conjugate) for sandwich imm...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"