Anti-Listeria monocytogenes抗体(Biotin) (ab20766)


  • 产品名称Anti-Listeria monocytogenes抗体(Biotin)
    参阅全部 Listeria monocytogenes 一抗
  • 描述
    兔多克隆抗体to Listeria monocytogenes (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Flow Cyt, ELISA, IHC-Frmore details
  • 种属反应性
    Antiserum is not absorbed and may react with other related microorganisms. Reacts with Group A Streptococcus, Group B Streptococcus, S. pneumoniae, Staph aureus, clostridium perfingens and Bacillus subtilis. Not yet tested in other species.
  • 免疫原

    Listeria monocytogenes, ATCC #43251

  • 常规说明

    IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab20766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab200208-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ELISA 1/1000.
IHC-Fr 1/250.


  • 相关性The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
  • 别名
    • L. monocytogenes antibody

Anti-Listeria monocytogenes antibody (Biotin) 图像

  • ab20766 staining Listeria monocytogenes in mouse spleen tissue section from a mice infected with 1x10exp6 L. monocytogenes for 24h by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 4% paraformaldehyde for 2 hours at RT and then it was incubated ON in 30% Sucrose in PBS at 4°C, froze away in OCT/Tissuetek. The tissue samples were blocked with 1% 1:20 Cd16/32 at 200C for 30 minutes. The sample was incubated with primary antibody (1/250) in PBS at 200C for 1 hour. For detection Streptavidin-Cy3 was used at dilution at 1:500 in PBS for 30 min at 20°C.

    See Abreview

  • Flow cytometry analysis of Listeria monocytogenes in culture, staining Listeria monocytogenes with ab20766.

    The sample was incubated with the primary antibody (1/300 in PBS) for 30 minutes at 4°C. A Streptavidin-Peridinin Chlorophyll-a Protein conjugated with PerCP was used for detection.

    Gating Strategy: FSC/SSC

    See Abreview

Anti-Listeria monocytogenes antibody (Biotin) (ab20766)参考文献

This product has been referenced in:
  • Dresing P  et al. A fluorescence reporter model defines "Tip-DCs" as the cellular source of interferon ß in murine listeriosis. PLoS One 5:e15567 (2010). IHC-Fr . Read more (PubMed: 21179567) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Sample Listeria monocytogenes Cell (Listeria monocytogenes in culture)
Specification Listeria monocytogenes in culture
Permeabilization No
Gating Strategy FSC/SSC

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Verified customer

提交于 Nov 22 2012


Ablaufdatum: 20. November 2012

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Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (Spleen)
Specification Spleen
Fixative Paraformaldehyde
Permeabilization No
Blocking step 1:20 Cd16/32, normal rat normal mouse as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Jul 17 2009