概述

  • 产品名称Anti-LIS1抗体
    参阅全部 LIS1 一抗
  • 描述
    山羊多克隆抗体to LIS1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    TGSVDQTVKVWECR

    , corresponding to C terminal amino acids 397-410 of Human LIS1.

  • 阳性对照
    • NIH-3T3 lysate
  • 常规说明Principal Names – PAFAH1B1; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45 kDa; LIS1; MDCR; PAFAH; lissencephaly 1 protein; Platelet-activating factor acetylhydrolase, isoform 1B, alpha subunit; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD) Official Gene Symbol - PAFAH1B1 GenBank Accession Number – NP_000421 LocusLink ID - 5048 (human) Gene Ontology terms - plasma protein; lipid metabolism; neurogenesis; cell motility; signal transduction; cytoplasm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab2656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 46.6 kDa).Can be blocked with Human LIS1 peptide (ab22930).
ICC/IF 1/200. PubMed: 18267077

靶标

  • 功能Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.
  • 组织特异性Fairly ubiquitous expression in both the frontal and occipital areas of the brain.
  • 疾病相关Defects in PAFAH1B1 are the cause of lissencephaly type 1 (LIS1) [MIM:607432]; also known as classic lissencephaly. LIS1 is characterized by agyria or pachgyria and disorganization of the clear neuronal lamination of normal six-layered cortex. The cortex is abnormally thick and poorly organized with 4 primitive layers. LIS1 is associated with enlarged and dysmorphic ventricles and often hypoplasia of the corpus callosum.
    Defects in PAFAH1B1 are the cause of subcortical band heterotopia (SBH) [MIM:607432]. SBH is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric ribbons of gray matter found in the central white matter between the cortex and the ventricular surface.
    Defects in PAFAH1B1 are a cause of Miller-Dieker lissencephaly syndrome (MDLS) [MIM:247200]. MDLS is a contiguous gene deletion syndrome of chromosome 17p13.3, characterized by classical lissencephaly and distinct facial features. Additional congenital malformations can be part of the condition.
  • 序列相似性Belongs to the WD repeat LIS1/nudF family.
    Contains 1 LisH domain.
    Contains 7 WD repeats.
  • 结构域Dimerization mediated by the LisH domain may be required to activate dynein.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Nucleus membrane. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LIS 1 antibody
    • LIS 2 antibody
    • LIS-1 antibody
    • LIS1 antibody
    • LIS1_HUMAN antibody
    • LIS2 antibody
    • Lissencephaly 1 protein antibody
    • Lissencephaly-1 protein antibody
    • MDCR antibody
    • MDS antibody
    • PAF acetylhydrolase 45 kDa subunit antibody
    • PAF AH 45 kDa subunit antibody
    • PAF AH alpha antibody
    • PAF-AH 45 kDa subunit antibody
    • PAF-AH alpha antibody
    • PAFAH alpha antibody
    • PAFAH antibody
    • PAFAH1B1 antibody
    • PAFAHA antibody
    • Platelet activating factor acetylhydrolase 1b regulatory subunit 1 antibody
    • Platelet activating factor acetylhydrolase isoform Ib alpha subunit antibody
    • Platelet-activating factor acetylhydrolase IB subunit alpha antibody
    see all

Anti-LIS1 antibody 图像

  • Anti-LIS1 antibody (ab2656) at 0.1 µg/ml + Rat ovary lysate in RIPA buffer. at 35 µg

    Predicted band size : 46.6 kDa


    Primary incubation for 1 hour. Detected by chemiluminescence.

Anti-LIS1 antibody (ab2656)参考文献

This product has been referenced in:
  • Yingling J  et al. Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division. Cell 132:474-86 (2008). ICC/IF ; Mouse . Read more (PubMed: 18267077) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% triton-X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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提交于 Jul 06 2011

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