重组Anti-Lipoprotein lipase抗体[EPR1555(2)] (ab172953)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1555(2)] to Lipoprotein lipase
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Lipoprotein lipase抗体[EPR1555(2)]
参阅全部 Lipoprotein lipase 一抗 -
描述
兔单克隆抗体[EPR1555(2)] to Lipoprotein lipase -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Guinea pig, Cat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal kidney, fetal heart, pancreas and fetal liver lysates. Human liver and pancreas tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1555(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172953于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 53 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. -
疾病相关
Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. -
序列相似性
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
翻译后修饰
Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. -
细胞定位
Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 4023 Human
- Omim: 609708 Human
- SwissProt: P06858 Human
- Unigene: 180878 Human
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别名
- EC 3.1.1 antibody
- EC 3.1.1.34 antibody
- HDLCQ11 antibody
see all
图片
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All lanes : Anti-Lipoprotein lipase antibody [EPR1555(2)] (ab172953) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human pancreas tissue lysate
Lane 4 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa -
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Lipoprotein lipase with ab172953 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Lipoprotein lipase with ab172953 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab172953 被引用在 1 文献中.
- Shen J et al. MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition. Aging (Albany NY) 12:15756-15770 (2020). PubMed: 32805717