Anti-Lipoprotein lipase抗体(ab86927)

概述

  • 产品名称Anti-Lipoprotein lipase抗体
    参阅全部 Lipoprotein lipase 一抗
  • 描述
    兔多克隆抗体to Lipoprotein lipase
  • 特异性No cross reactivity with other proteins is observed.
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence at the C-terminal of Human Lipoprotein lipase, different to the related rat sequence by two amino acids.

  • 阳性对照
    • MCF7, HeLa, Jurkat and Smmc whole cell lystes. IHC-P: Human heart FFPE tissue sections. IF/ICC: HeLa cell line

性能

应用

Our Abpromise guarantee covers the use of ab86927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
  • 疾病相关Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.
  • 序列相似性Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.
  • 翻译后修饰Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.
  • 细胞定位Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 3.1.1 antibody
    • EC 3.1.1.34 antibody
    • HDLCQ11 antibody
    • LIPD antibody
    • LIPL_HUMAN antibody
    • Lipoprotein lipase antibody
    • LPL antibody
    • LPL protein antibody
    • MGC137861 antibody
    see all

Anti-Lipoprotein lipase antibody 图像

  • ICC/IF image of ab86927 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86927, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of Lipoprotein lipase staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86927, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Lipoprotein lipase antibody (ab86927) at 1 µg/ml

    Lane 1 : Milk
    Lane 2 : Milk
    Lane 3 : MCF7 whole cell lysate
    Lane 4 : HeLa whole cell lysate
    Lane 5 : Jurkat whole cell lysate
    Lane 6 : Smmc whole cell lysate


    Predicted band size : 53 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <29 kDa. We are unsure as to the identity of these extra bands.
    N.B. There are two gylcosylation sites on the protein.

Anti-Lipoprotein lipase antibody (ab86927)参考文献

ab86927 has not yet been referenced specifically in any publications.

Product Wall


Yes, I can definitely send you a replacement vial free of charge. We have another lot for this antibody in stock. Alternatively, a different antibody to LPL can be sent.

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