Anti-Lipocalin-2 / NGAL抗体[ABS 039-08] (ab53500)

概述

  • 产品名称Anti-Lipocalin-2 / NGAL抗体[ABS 039-08]
    参阅全部 Lipocalin-2 / NGAL 一抗
  • 描述
    小鼠单克隆抗体[ABS 039-08] to Lipocalin-2 / NGAL
  • 特异性This antibody reacts with Lipocalin-2 / NGAL
  • 经测试应用适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Rat
    不与反应: Human
  • 免疫原

    Recombinant full length protein (His-tag) corresponding to Rat Lipocalin-2/ NGAL (C terminal). His-tag coupled to carrier

  • 常规说明FUSION PARTNER: Sp2/mIL-6.

性能

应用

Our Abpromise guarantee covers the use of ab53500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/1000.

靶标

  • 功能Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • 组织特异性Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
  • 细胞定位Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 24p3 antibody
    • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
    • Alpha 2 microglobulin related protein antibody
    • HGNC:6526 antibody
    • HNL antibody
    • Lcn 2 antibody
    • Lcn2 antibody
    • Lipocalin-2 antibody
    • Migration stimulating factor inhibitor antibody
    • MSFI antibody
    • Neutrophil gelatinase associated lipocalin antibody
    • Neutrophil gelatinase associated lipocalin precursor antibody
    • Neutrophil gelatinase-associated lipocalin antibody
    • NGAL antibody
    • NGAL_HUMAN antibody
    • Oncogene 24p3 antibody
    • Oncogenic lipocalin 24p3 antibody
    • p25 antibody
    • Siderocalin antibody
    • siderocalin LCN2 antibody
    • SV40 induced 24P3 protein antibody
    • Uterocalin antibody
    see all

Anti-Lipocalin-2 / NGAL antibody [ABS 039-08] 图像

  • Lipocalin-2 / NGAL immunostaining in kidney, Formalin-fixed, paraffin-embedded tissue sections of cortical epithelial cells in the S1/S2 segments (proximal convoluted tubules) following DXR-induced acute kidney injury. Top panel, saline treatment; bottom panel, DXR treatment; X630 (2).

Anti-Lipocalin-2 / NGAL antibody [ABS 039-08] (ab53500)参考文献

ab53500 has not yet been referenced specifically in any publications.

Product Wall

I was able to obtain the protocol used by the scientists who made the images on the datasheet. Antigen retrieval is required and primary antibody incubation was done at 1:50. I have attached this protocol in pdf format for your review.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"