Anti-Lipocalin-2 / NGAL抗体(ab41105)

概述

  • 产品名称Anti-Lipocalin-2 / NGAL抗体
    参阅全部 Lipocalin-2 / NGAL 一抗
  • 描述
    兔多克隆抗体to Lipocalin-2 / NGAL
  • 特异性A customer has submitted a positive Abreview for rat samples in IHC. However, customers have experienced problems with rat samples and this is likely due to the low homology between the immunogen and the rat protein (30%). Some batches of this antibody may work in rat, but this is not batch tested.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Lipocalin-2/ NGAL aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab41759, ab41759, ab41759, ab41759, ab41758, ab41758, ab41758, ab41758, ab41758)

  • 阳性对照
    • ab41105 gave a positive result in Human Colon Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab41105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 5 µg/ml.
    IHC-P: Use at a dilution of 1/250-1/1000 for 1 hr.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
    • 组织特异性Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
    • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
    • 细胞定位Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 24p3 antibody
      • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
      • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
      • Alpha 2 microglobulin related protein antibody
      • HGNC:6526 antibody
      • HNL antibody
      • Lcn 2 antibody
      • Lcn2 antibody
      • Lipocalin-2 antibody
      • Migration stimulating factor inhibitor antibody
      • MSFI antibody
      • Neutrophil gelatinase associated lipocalin antibody
      • Neutrophil gelatinase associated lipocalin precursor antibody
      • Neutrophil gelatinase-associated lipocalin antibody
      • NGAL antibody
      • NGAL_HUMAN antibody
      • Oncogene 24p3 antibody
      • Oncogenic lipocalin 24p3 antibody
      • p25 antibody
      • Siderocalin antibody
      • siderocalin LCN2 antibody
      • SV40 induced 24P3 protein antibody
      • Uterocalin antibody
      see all

    Anti-Lipocalin-2 / NGAL antibody 图像

    • Anti-Lipocalin-2 / NGAL antibody (ab41105) at 1 µg/ml + Human colon tissue lysate - total protein (ab30051) at 30 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 23 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 25 kDa,70 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 12 minutes
    • ICC/IF image of ab41105 stained human Hek293 cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41105, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • ab41105 staining Lipocalin-2 / NGAL in human lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes peroxidase block and 10 minutes protein blocks). The primary antibody was diluted 1/250 or 1/1000 and incubated  with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as the secondary.   

      See Abreview

    • ab41105 staining Lipocalin-2 / NGAL in rat lung tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes peroxidase block and 10 minutes protein blocks). The primary antibody was diluted 1/250 or 1/1000 and incubated  with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as the secondary. 

      See Abreview

    Anti-Lipocalin-2 / NGAL antibody (ab41105)参考文献

    This product has been referenced in:
    • Bienholz A  et al. Adverse effects of a-ketoglutarate/malate in a rat model of acute kidney injury. Am J Physiol Renal Physiol 303:F56-63 (2012). IHC-P ; Rat . Read more (PubMed: 22513847) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human sputum)
    Loading amount 20 µg
    Specification human sputum
    Gel Running Conditions Reduced Denaturing (15%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Mr. Taehyeong lee

    Verified customer

    提交于 Nov 16 2012

    Thank you for contacting us. I am sorry that these antibodies are not giving any signal in IHC.

    Have you been able to use the secondary antibody successfully with any other primaries? If not, I would recommend spotting the secondary and DAB r...

    Read More

    Thank you for taking time to answer my questions. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monito...

    Read More

    Thank you for taking time to send us your protocol. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for moni...

    Read More

    Thank you for providing that information. This will be very useful in investigating your complaint further.


    If you could provide me with the order number on which the antibody was ordered (or the approximate delivery date and delivery a...

    Read More
    Application Western blot
    Sample Rat Tissue lysate - whole (Lung)
    Loading amount 50 µg
    Specification Lung
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 17 2011

    Application Western blot
    Sample Rat Cell lysate - whole cell (Hepatocytes)
    Loading amount 50 µg
    Specification Hepatocytes
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Shakil Ahmad

    Verified customer

    提交于 Aug 09 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (lung)
    Specification lung
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    提交于 Oct 28 2008

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (lung)
    Specification lung
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    提交于 Oct 28 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"