Anti-Lipocalin-2 / NGAL抗体[5G5] (ab23477)


  • 产品名称Anti-Lipocalin-2 / NGAL抗体[5G5]
    参阅全部 Lipocalin-2 / NGAL 一抗
  • 描述
    小鼠单克隆抗体[5G5] to Lipocalin-2 / NGAL
  • 特异性ab23477 is specific for Lipocalin-2 / NGAL, native and denatured forms.
  • 经测试应用适用于: IHC-P, ELISA, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Lipocalin-2/ NGAL. Full length native human neutrophil gelatinase associated lipocalin isolated from neutrophils.

  • 阳性对照
    • Capa-2, BxPC-3, ASPC1 cell line



Our Abpromise guarantee covers the use of ab23477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10. PubMed: 18317594
ELISA 1/30000.
IHC-FoFr 1/10.
WB 1/1000. Detects a band of approximately 25-35 kDa (predicted molecular weight: 23 kDa). Non reducing conditions will give multiple bands of 35, 70 and 55 kDa. Do not use serum for blocking as serum will contain Lipocalin 2


  • 功能Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • 组织特异性Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
  • 细胞定位Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 24p3 antibody
    • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
    • Alpha 2 microglobulin related protein antibody
    • HGNC:6526 antibody
    • HNL antibody
    • Lcn 2 antibody
    • Lcn2 antibody
    • Lipocalin-2 antibody
    • Migration stimulating factor inhibitor antibody
    • MSFI antibody
    • Neutrophil gelatinase associated lipocalin antibody
    • Neutrophil gelatinase associated lipocalin precursor antibody
    • Neutrophil gelatinase-associated lipocalin antibody
    • NGAL antibody
    • NGAL_HUMAN antibody
    • Oncogene 24p3 antibody
    • Oncogenic lipocalin 24p3 antibody
    • p25 antibody
    • Siderocalin antibody
    • siderocalin LCN2 antibody
    • SV40 induced 24P3 protein antibody
    • Uterocalin antibody
    see all

Anti-Lipocalin-2 / NGAL antibody [5G5] 图像

  • ab23477 staining human skin tissue sections by IHC-P.  Sections were fixed in 10% neutral buffered formalin and subjected to heat mediated antigen retrieval prior to blocking with 1% BSA for 1 hour at 25°C.  The primary antibody was diluted 1/50 and incubated with the sample for 20 hours at 4°C.  A biotinylated horse anti-mouse antibody diluted 1/200 was used as the secondary.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human PaCa tissue sections labelling Lipocalin 2 with ab23477.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human chronic pancreatits tissue sections labelling Lipocalin-2 / NGAL with ab23477.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human pancreas tissue sections labelling Lipocalin-2 / NGAL with ab23477.

Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)参考文献

This product has been referenced in:
  • Wolk K  et al. Deficient cutaneous antibacterial competence in cutaneous T-cell lymphomas: role of Th2-mediated biased Th17 function. Clin Cancer Res 20:5507-16 (2014). Read more (PubMed: 25212608) »
  • Chung TW  et al. Lipocalin-2 elicited by advanced glycation end-products promotes the migration of vascular smooth muscle cells. Biochim Biophys Acta 1833:3386-95 (2013). Human . Read more (PubMed: 24149112) »

See all 11 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human sebacous gland cells)
Loading amount 60 µg
Specification human sebacous gland cells
Treatment 13-cis Retinoic acid for 48 hours
Gel Running Conditions Reduced Denaturing (4- 12% Bis-Tris gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 May 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human skin sections)
Specification Human skin sections
Fixative 10% Neutral Buffered Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Trilogy (Cell Marque)
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Apr 22 2008