Anti-Lipocalin-2 / NGAL抗体[29] (ab70287)

概述

  • 产品名称
    Anti-Lipocalin-2 / NGAL抗体[29]
    参阅全部 Lipocalin-2 / NGAL 一抗
  • 描述
    大鼠单克隆抗体[29] to Lipocalin-2 / NGAL
  • 特异性
    ab70287 binds Lipocalin-2 / NGAL in mouse serum.
  • 经测试应用
    适用于: WB, ELISA, IHC-P, Sandwich ELISAmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant full length protein corresponding to Mouse Lipocalin-2/ NGAL. Recombinant mouse Lipocalin-2/ NGAL adsorbed onto aluminum hydroxide gel

性能

应用

Our Abpromise guarantee covers the use of ab70287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ELISA 1/4000.
IHC-P Use at an assay dependent concentration.

Microwave pretreatment using citrate buffer pH 6, is recommended.

Sandwich ELISA Use at an assay dependent concentration. Can be used as Detection antibody with recommended pair.

靶标

  • 功能
    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • 组织特异性
    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • 序列相似性
    Belongs to the calycin superfamily. Lipocalin family.
  • 细胞定位
    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 24p3 antibody
    • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
    • Alpha 2 microglobulin related protein antibody
    • HGNC:6526 antibody
    • HNL antibody
    • Lcn 2 antibody
    • Lcn2 antibody
    • Lipocalin-2 antibody
    • Migration stimulating factor inhibitor antibody
    • MSFI antibody
    • Neutrophil gelatinase associated lipocalin antibody
    • Neutrophil gelatinase associated lipocalin precursor antibody
    • Neutrophil gelatinase-associated lipocalin antibody
    • NGAL antibody
    • NGAL_HUMAN antibody
    • Oncogene 24p3 antibody
    • Oncogenic lipocalin 24p3 antibody
    • p25 antibody
    • Siderocalin antibody
    • siderocalin LCN2 antibody
    • SV40 induced 24P3 protein antibody
    • Uterocalin antibody
    see all

Anti-Lipocalin-2 / NGAL antibody [29] 图像

  • Lipocalin-2 / NGAL expression in the ischemia-reperfusion injured mouse kidney; detected with anti-mouse Lipocalin-2 / NGAL antibody (ab70287) (1:1000).

  • Lipocalin-2 / NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney; detected with ab70287 at 1/1000.

Anti-Lipocalin-2 / NGAL antibody [29] (ab70287)参考文献

ab70287 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 14 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formalin
Username

Abcam user community

Verified customer

提交于 Mar 10 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册