概述

  • 产品名称Anti-Lipin 3抗体
    参阅全部 Lipin 3 一抗
  • 描述
    兔多克隆抗体to Lipin 3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Lipin 3.

  • 阳性对照
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab70159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Regulates fatty acid metabolism. Magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis.
  • 组织特异性Significant expression in intestine and other regions of the gastrointestinal tract.
  • 序列相似性Belongs to the lipin family.
  • 结构域Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif known to be essential for phosphatidate phosphatase activity.
    Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL) motif, a motif known to be a transcriptional binding motif.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ450M14.2 antibody
    • dJ450M14.3 antibody
    • dJ620E11.2 antibody
    • Lipin 3 like antibody
    • Lipin-3 antibody
    • Lipin-3-like antibody
    • Lipin3 antibody
    • LIPN3L antibody
    • LPIN 3 antibody
    • Lpin3 antibody
    • LPIN3_HUMAN antibody
    • Phosphatidate phosphatase LPIN3 antibody
    • RP4 620E11.3 antibody
    • SMP2 antibody
    see all

Anti-Lipin 3 antibody 图像

  • Anti-Lipin 3 antibody (ab70159) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 94 kDa
    Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,45 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab70159 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70159, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Lipin 3 antibody (ab70159)参考文献

ab70159 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70159.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"