概述

  • 产品名称
    Anti-Lipin 1抗体
    参阅全部 Lipin 1 一抗
  • 描述
    兔多克隆抗体to Lipin 1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human Lipin 1.

    (Peptide available as ab86631.)

  • 阳性对照
    • This antibody gave a positive signal in the following Mouse Tissue Lysates: Liver; Pancreas, Kidney.

性能

应用

Our Abpromise guarantee covers the use of ab70138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).

靶标

  • 功能
    Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
  • 组织特异性
    Abundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract.
  • 疾病相关
    Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years.
  • 序列相似性
    Belongs to the lipin family.
  • 结构域
    Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
    Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
  • 翻译后修饰
    Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
    Sumoylated.
  • 细胞定位
    Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC=3.1.3.4 antibody
    • KIAA0188 antibody
    • Lipin-1 antibody
    • Lpin1 antibody
    • LPIN1_HUMAN antibody
    • PAP1 antibody
    • Phosphatidate phosphatase LPIN1 antibody
    see all

图片

  • All lanes : Anti-Lipin 1 antibody (ab70138) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate
    Lane 3 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Cai K  et al. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 1841:552-62 (2014). WB . Read more (PubMed: 24369117) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Airway epithelial cells)
Specification
Airway epithelial cells
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Prof. Marc Chanson

Verified customer

提交于 Sep 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Loading amount
30 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
50 µg
Specification
mouse heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Apr 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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