概述

  • 产品名称
    Anti-Lipid A抗体
    参阅全部 Lipid A 一抗
  • 描述
    山羊多克隆抗体to Lipid A
  • 特异性
    The antibody was tested in direct IF on cells fixed with acetone and the secondary antibody was labeled with FITC.
  • 经测试应用
    适用于: Conjugation, ICC/IFmore details
  • 种属反应性
    Reactive with numerous members of the Enterobacteriaciae. Pseudomonas aeruginosa, Klebsiella pneumoniae, E. coli O157, Salmonella enteriditis, Enterobacter aerogenes, E. hermanii, Yersinia enterocolitica and Shigella sonnei. Not yet tested in other species.
  • 免疫原

    Whole cells prep of Lipid A from E. coli O157.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    Sodium sulfate precipitation and ion-exchange chromatography.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Conjugation Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution. Fix with acetone.

靶标

  • 相关性
    Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.
  • 细胞定位
    Secreted

Anti-Lipid A antibody (ab20001)参考文献

This product has been referenced in:
  • Jensen HH  et al. Tir Is Essential for the Recruitment of Tks5 to Enteropathogenic Escherichia coli Pedestals. PLoS One 10:e0141871 (2015). ICC/IF . Read more (PubMed: 26536015) »
  • Miyata ST  et al. Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun 79:2941-9 (2011). Read more (PubMed: 21555399) »

See all 2 Publications for this product

Product Wall

Application
ELISA
Sample
Mouse Serum (also plasma)
Specification
also plasma
Type
Other
Blocking step
Pierce protein free buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 14 2014

Abreviews
Application
Western blot
Sample
Acinetobacter baumannii Cell lysate - whole cell (bacteria)
Loading amount
300000 cells
Specification
bacteria
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 09 2012

Abreviews
Application
Western blot
Sample
Pseudomonas aeruginosa Cell lysate - whole cell (bacteria)
Loading amount
300000 cells
Specification
bacteria
Gel Running Conditions
Non-reduced Denaturing (gel 12%)
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 05 2012

Code de remise : XXX%XXX-XXXX Date d’expiration : JJ MM AAAA Je suis ravi que vous souhaitiez participer à notre offre et tester ab20001 chez Acinetobacter baumannii. Le code de réduction correspondant est 100%ABR-E7TFB. Ce code de réduction ...

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Thank you for contacting us. Lab let me know that an affinity constant of Catalog ab20001, Goat anti Lipid A, has not been determined. This antibody recognizes the lipid A portion of LPS . When tested by IFA, the antibody shows strongest reac...

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Thank you for your enquiry. The antibody was tested in direct IF on cells fixed with acetone and the secondary antibody was labeled with FITC. I have added this information to the online datasheet. I hope this information helps, please do not he...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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