The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).
组织特异性Expressed in testis. Expressed exclusively at the connecting piece of the sperm.
疾病相关Defects in LIPI may be a cause of susceptibility to familial hypertriglyceridemia (FHTR)[MIM:145750]. Familial hypertriglyceridemia is a common inherited disorder in which the concentration of very low density lipoprotein (VLDL) is elevated in the plasma. This leads to increased risk of heart disease, obesity, and pancreatitis.
序列相似性Belongs to the AB hydrolase superfamily. Lipase family.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Membrane. May associate with lipid draft and Membrane. Secreted. May associate with lipid draft.