概述

  • 产品名称Anti-LIPG抗体
    参阅全部 LIPG 一抗
  • 描述
    小鼠单克隆抗体to LIPG
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 21-501 of Human LIPG

性能

应用

Our Abpromise guarantee covers the use of ab56493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
    • 组织特异性High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocyte and osteosarcoma cell lines. Not detected in heart, brain and muscle.
    • 序列相似性Belongs to the AB hydrolase superfamily. Lipase family.
      Contains 1 PLAT domain.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EDL antibody
      • EL antibody
      • Endothelial cell-derived lipase antibody
      • Endothelial lipase antibody
      • Endothelial lipase precursor antibody
      • Lipase endothelial antibody
      • LIPE_HUMAN antibody
      • LIPG antibody
      • Lipoprotein lipase H antibody
      • PRO719 antibody
      see all

    Anti-LIPG antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab56493 LIPG antibody at 1ug/ml. Predicted band size of immunogen is 79 kDa
    • Anti-LIPG antibody (ab56493) at 1/1000 dilution + whole cell lysate prepared from human HUVEC cells at 25 µg

      Secondary
      Rabbit anti-mouse IRDye 800CW at 1/5000 dilution

      Performed under reducing conditions.

      Observed band size : 80 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 minutes

      This image is courtesy of an anonymous abreview.

      See Abreview

    Anti-LIPG antibody (ab56493)参考文献

    ab56493 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HUVEC)
    Loading amount 25 µg
    Specification HUVEC
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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    提交于 Dec 29 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"