概述

  • 产品名称
  • 描述
    兔多克隆抗体to LIPG
  • 特异性
    ab24447 reacts with Endothelial lipase.
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Human, Pig
  • 免疫原

    Synthetic peptide surrounding amino acid 22 of human Endothelial Lipase. (Peptide available as ab53323.)

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab24447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 - 20 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 57 kDa.Can be blocked with LIPG peptide (ab53323).

靶标

  • 功能
    Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
  • 组织特异性
    High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocyte and osteosarcoma cell lines. Not detected in heart, brain and muscle.
  • 序列相似性
    Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EDL antibody
    • EL antibody
    • Endothelial cell-derived lipase antibody
    • Endothelial lipase antibody
    • Endothelial lipase precursor antibody
    • Lipase endothelial antibody
    • LIPE_HUMAN antibody
    • LIPG antibody
    • Lipoprotein lipase H antibody
    • PRO719 antibody
    see all

图片

  • Anti-LIPG antibody (ab24447) + HepG2 lysate

    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab24447 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24447, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Kimura N  et al. Multiple endocrine neoplasia type 1-associated cystic pancreatic endocrine neoplasia and multifocal cholesterol granulomas. Pathol Int 60:321-5 (2010). IHC-P ; Human . Read more (PubMed: 20403035) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Nov 05 2014

Thank you for your enquiry. I am afraid that we do not have any information on expression in pancreas. However, based on our laboratory testing, we did find staining in human tyroid sections. Also, according to this publication, lipase is also expr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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