antigen, corresponding to amino acids 439-537 of Human LINS Isoform 1 (Q8NG48).
Shipped at 4°C. Store at -20°C.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Expresssed in adult testis, prostate, prostate, spleen, thymus, skeletal muscle, fetal kidney and brain.
Belongs to the protein lines family.
Information by UniProt
- LINES_HUMAN antibody
- LINS antibody
- Protein Lines homolog 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-LINS antibody (ab122488)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122488 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LINS antibody (ab122488)
ab122488, at 1/50 dilution, staining LINS in formalin-fixed, paraffin-embedded Human cerebellum tissue.
has not yet been referenced specifically in any publications.
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