概述

  • 产品名称Anti-Lingo1抗体
    参阅全部 Lingo1 一抗
  • 描述
    兔多克隆抗体to Lingo1
  • 特异性Rabbit polyclonal to Lingo1 (ab23631) detects Lingo1 protein at ~83kDa in mouse and human brain lysates. This band is larger than predicted on Swiss Prot (69kDa; Q9D1T0) possibly due to post-translational modification and is consistent with published literature on Lingo1 protein detection in brain lysate. The strong band observed at ~ 17kDa in the mouse brain lysate (lane 1) corresponds to a cleavage fragment of Lingo1 (Swiss Prot IDs: Q3TQJ4)
  • 经测试应用适用于: WB, ICC/IF, IHC-Fr, IP, Functional Studies, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human Lingo1.

    (Peptide available as ab25890.)

  • 阳性对照
    • Mouse Brain, Brain (Mouse) Whole Cell Lysate - normal tissue, 0 days old (ab7188) This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.

性能

应用

Our Abpromise guarantee covers the use of ab23631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/500. PubMed: 20407577
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. PubMed: 20573699
IHC-FoFr 1/3000.

靶标

  • 功能Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development.
  • 组织特异性Expressed exclusively in the central nervous system. Highest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression seems to be always present. Up-regulated in substantia nigra neurons from Parkinson disease patients.
  • 序列相似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • 翻译后修饰N-glycosylated. Contains predominantly high-mannose glycans.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ14594 antibody
    • LERN 1 antibody
    • LERN1 antibody
    • Leucine rich repeat and Ig domain containing 1 antibody
    • Leucine rich repeat neuronal 6A antibody
    • Leucine rich repeat neuronal protein 1 antibody
    • Leucine rich repeat neuronal protein 6A antibody
    • Leucine-rich repeat and immunoglobilin domain-containing protein 1 antibody
    • Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 antibody
    • Leucine-rich repeat neuronal protein 1 antibody
    • Leucine-rich repeat neuronal protein 6A antibody
    • LIGO1_HUMAN antibody
    • Lingo 1 antibody
    • LINGO1 antibody
    • LRR and Ig domain containing Nogo Receptor interating protein antibody
    • Lrrn 6a antibody
    • Lrrn6a antibody
    • Lrrn6a protein antibody
    • MGC17422 antibody
    • Nogo Receptor interacting protein antibody
    • PRO227 antibody
    • UNQ201 antibody
    see all

Anti-Lingo1 antibody 图像

  • ICC/IF image of ab23631 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23631 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Lingo1 antibody (ab23631) at 1 µg/ml

    Lane 1 : Mouse brain
    Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 3 : Human Brain Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
    Additional bands at : 17 kDa (possible cleavage fragment),34 kDa (possible degradation product).

    Rabbit polyclonal to Lingo1 (ab23631) detects Lingo1 protein at ~83kDa in mouse and human brain lysates. This band is larger than predicted on Swiss Prot (69kDa; Q9D1T0) possibly due to post-translational modifications and is consistent with published literature on Lingo1 protein detection in brain lysate. The strong band observed at ~ 17kDa in the mouse brain lysate (lane 1) corresponds to a cleavage fragment of Lingo1 (Swiss Prot IDs: Q3TQJ4)

    See Abreview

  • Lingo1 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Lingo1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23631.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 83kDa:Lingo1; non specific - 70kDa: We are unsure as to the identity of this extra band.
  • ab23631 staining Lingo1 in in rat brain tissue by Immunohistochemistry (Frozen sections). Rats were decapitated, brains were quickly removed and immediately frozen in isopentane (at -30°C for 3 minutes) and kept at -25°C. Coronal sections were cut at 14 µm in a cryostat and placed on gelatinized glass slides, air-dried at room temperature for 20 minutes and kept at -25°C until further processing. Brain sections were fixed for 10 minutes in cold acetone and washed three times in 1 × phosphate-buffered saline (PBS). Non-specific binding sites were blocked by incubating slices for 1 hour in 1 × PBS containing 1% bovine serum albumin, 1% Triton X-100 and 3% normal goat serum. Sections were then incubated overnight at 4°C with the primary antibody at a 1/500 dilution. The sections were then stained by 30 minutes incubation with Hoechst at a 1/1500 dilution.
  • ICC/IF image of ab23631 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23631 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Lingo1 antibody (ab23631)参考文献

This product has been referenced in:
  • Fernandez-Enright F  et al. Novel implications of Lingo-1 and its signaling partners in schizophrenia. Transl Psychiatry 4:e348 (2014). WB ; Human . Read more (PubMed: 24448210) »
  • Kwon HS  et al. Myocilin is involved in NgR1/Lingo-1-mediated oligodendrocyte differentiation and myelination of the optic nerve. J Neurosci 34:5539-51 (2014). Read more (PubMed: 24741044) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Brain protein lysate)
Loading amount 30 µg
Specification Brain protein lysate
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Top Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Mr. Marija Petrinovic

Verified customer

提交于 Sep 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"