概述

  • 产品名称
  • 描述
    兔多克隆抗体to Lin28B
  • 经测试应用
    适用于: ICC/IF, IHC-P, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide between amino acids of 105-135 of human Lin28B, conjugated to KLH. The peptide has 80% homology for LIN28A (Q9H9Z2).

  • 阳性对照
    • WB: K562 cell line lysates. IHC-P: Human testis and spleen tissues. ICC/IF: HepG2 cells. Flow Cyt: HL-60 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/10 - 1/50.
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

靶标

  • 功能
    Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
  • 组织特异性
    High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level).
  • 序列相似性
    Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • 细胞定位
    Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSDD 2 antibody
    • CSDD2 antibody
    • FLJ16517 antibody
    • Lin 28 homolog B (C. elegans) antibody
    • Lin 28.2 antibody
    • Lin-28B antibody
    • LIN28B antibody
    • LN28B_HUMAN antibody
    • Protein lin-28 homolog B antibody
    see all

Anti-Lin28B antibody 图像

  • Anti-Lin28B antibody (ab71415) at 1/1000 dilution + K562 cell line lysates at 35 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical staining of Lin28B in Human testis tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab71415 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human spleen tissue labelling Lin28B with ab71415 at 1/50. A peroxidase conjugated secondary antibody was used followed by AEC staining.

  • Immunocytochemisty/Immunofluorescence analysis of HepG2 cells labelling Lin28B (green) with ab71415. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstained with DAPI (blue)

  • Flow cytometry analysis of HL-60 cells labelling Lin28B (green) with ab71415 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Anti-Lin28B antibody (ab71415)参考文献

This product has been referenced in:
  • Park SJ  et al. MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B. Oncogene 35:1292-301 (2016). IHC ; Human . Read more (PubMed: 26028027) »
  • Choi SA  et al. Disulfiram modulates stemness and metabolism of brain tumor initiating cells in atypical teratoid/rhabdoid tumors. Neuro Oncol 17:810-21 (2015). Read more (PubMed: 25378634) »

See all 11 Publications for this product

Product Wall

Te agradezco mucho el envío de los cuestionarios rellenos.

Hay un par de sugerencias que me gustaría hacerte para intentar mejorar la tinción de estos anticuerpos en vuestras muestras. De cualquier forma, siendo que la ga...

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Application
Western blot
Sample
Human Cell lysate - whole cell (GASTRIC CANCER CELL)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
50 µg
Specification
GASTRIC CANCER CELL
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: RT°C
Username

Mr. JUNGHO YOON

Verified customer

提交于 Aug 16 2017

DISCOUNT CODE: ***
Expiration date: December 14th, 2012

I am very pleased to hear you would like to accept our offer and test ab71415 in mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this o...

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I am sorry but we are unfortunately not able to provide sample sizes of any of our products. This is due to the logistics that would be involved in this, with such a large catalogue this simply isn't feasible...

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Thank you for contacting us yesterday.

As discussed over the phone, I have had a look into the two antibodies you have been using (ab46020 and ab71415).

As we discussed, the anti-Lin28 antibody (ab46020) has been raised against the ...

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Thank you for contacting us. Your credit note ID is xxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice ...

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Thank you for your reply. The testing discount details are below. When you come to use the discount code using your online software, If there is not a place to put the code (you can enter the code in the notes sections if you have too), then please cal...

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Thank you very much for your interest in ab71415. To our knowledge, ab71415has not been tested in mouse tissue. Therefore, I can offer a discount off a future purchase if you buy ab71415 now, test it in mouse tissue and submit feedback to us in the for...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human ES)
Total protein in input
800 µg
Specification
human ES
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

提交于 Nov 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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