概述

  • 产品名称Anti-Lin28B抗体
    参阅全部 Lin28B 一抗
  • 描述
    兔多克隆抗体to Lin28B
  • 经测试应用适用于: ICC/IF, IHC-P, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide between amino acids of 105-135 of human Lin28B, conjugated to KLH. The peptide has 80% homology for LIN28A (Q9H9Z2).

  • 阳性对照
    • WB: K562 cell line lysates. IHC-P: Human testis and spleen tissues. ICC/IF: HepG2 cells. Flow Cyt: HL-60 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/10 - 1/50.
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

靶标

  • 功能Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
  • 组织特异性High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level).
  • 序列相似性Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSDD 2 antibody
    • CSDD2 antibody
    • FLJ16517 antibody
    • Lin 28 homolog B (C. elegans) antibody
    • Lin 28.2 antibody
    • Lin-28B antibody
    • LIN28B antibody
    • LN28B_HUMAN antibody
    • Protein lin-28 homolog B antibody
    see all

Anti-Lin28B antibody 图像

  • Anti-Lin28B antibody (ab71415) at 1/1000 dilution + K562 cell line lysates at 35 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical staining of Lin28B in Human testis tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab71415 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human spleen tissue labelling Lin28B with ab71415 at 1/50. A peroxidase conjugated secondary antibody was used followed by AEC staining.

  • Immunocytochemisty/Immunofluorescence analysis of HepG2 cells labelling Lin28B (green) with ab71415. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstained with DAPI (blue)

  • Flow cytometry analysis of HL-60 cells labelling Lin28B (green) with ab71415 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Anti-Lin28B antibody (ab71415)参考文献

This product has been referenced in:
  • Choi SA  et al. Disulfiram modulates stemness and metabolism of brain tumor initiating cells in atypical teratoid/rhabdoid tumors. Neuro Oncol 17:810-21 (2015). Read more (PubMed: 25378634) »
  • Pang M  et al. LIN28B promotes colon cancer migration and recurrence. PLoS One 9:e109169 (2014). WB, IHC ; Human . Read more (PubMed: 25360631) »

See all 10 Publications for this product

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Expiration date: December 14th, 2012

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As discussed over the phone, I have had a look into the two antibodies you have been using (ab46020 and ab71415).

As we discussed, the anti-Lin28 antibody (ab46020) has been raised against the ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (human ES)
Total protein in input 800 µg
Specification human ES
Immuno-precipitation step Protein A/G
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提交于 Nov 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"