Anti-Lin28B抗体(ab115698)

概述

  • 产品名称Anti-Lin28B抗体
    参阅全部 Lin28B 一抗
  • 描述
    兔多克隆抗体to Lin28B
  • 经测试应用适用于: WB, ELISA, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH from a region corresponding to the N-terminal of Human Lin28B.

  • 阳性对照
    • Human spleen and testis tissues and HL60 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab115698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 27 kDa.
ELISA 1/1000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
  • 组织特异性High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level).
  • 序列相似性Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSDD 2 antibody
    • CSDD2 antibody
    • FLJ16517 antibody
    • Lin 28 homolog B (C. elegans) antibody
    • Lin 28.2 antibody
    • Lin-28B antibody
    • LIN28B antibody
    • LN28B_HUMAN antibody
    • Protein lin-28 homolog B antibody
    see all

Anti-Lin28B antibody 图像

  • Immunohistochemistry Image of Human Spleen (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry Image of Human Testis (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry Image of Human Testis (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-Lin28B antibody (ab115698) at 1/1000 dilution + HL60 cell line lysate at 35 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/5000 dilution

    Predicted band size : 27 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Overlay histogram showing HL-60 cells stained with ab115698 (green line). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab115698, 1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody used was FITC conjugated goat anti-rabbit IgG at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions. Acquisition of  >10,000 events was performed.

Anti-Lin28B antibody (ab115698)参考文献

ab115698 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.


The fixation for this image was 2% PFA at room temperature for 10 minutes. The permeabilization was done with 90% methanol.

I hope this information is helpful to you. Please do not hesitate to co...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"