概述

  • 产品名称Anti-Lin28A抗体
    参阅全部 Lin28A 一抗
  • 描述
    兔多克隆抗体to Lin28A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to a region surrounding amino acids 1-5 (MGSVS) of Human Lin28A (NP_078950.1).

  • 阳性对照
    • HepG2 cell extract

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab119392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).

靶标

  • 功能Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • 组织特异性Expressed in embryonic stem cells (ES cells), placenta and testis.
  • 序列相似性Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • 发展阶段Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • 结构域The CSD domain is required for function in muscle differentiation.
  • 细胞定位Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSDD1 antibody
    • FLJ12457 antibody
    • LIN 28 antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin-28A antibody
    • LIN28 antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • ZCCHC1 antibody
    • zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Anti-Lin28A antibody 图像

  • Anti-Lin28A antibody (ab119392) at 1/500 dilution + HepG2 cell extract

    Predicted band size : 23 kDa

Anti-Lin28A antibody (ab119392)参考文献

ab119392 has not yet been referenced specifically in any publications.

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