Anti-Lin28抗体[EPR4639] (ab109751)


  • 产品名称Anti-Lin28抗体[EPR4639]
    参阅全部 Lin28 一抗
  • 描述
    兔单克隆抗体[EPR4639] to Lin28
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of Human Lin28A.

  • 阳性对照
    • JAR and NCCIT cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
    • 组织特异性Expressed in embryonic stem cells (ES cells), placenta and testis.
    • 序列相似性Belongs to the lin-28 family.
      Contains 2 CCHC-type zinc fingers.
      Contains 1 CSD (cold-shock) domain.
    • 发展阶段Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
    • 结构域The CSD domain is required for function in muscle differentiation.
    • 细胞定位Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AL024421 antibody
      • CSDD1 antibody
      • CSDD2 antibody
      • FLJ12457 antibody
      • Lin 28 antibody
      • Lin 28 homolog (C. elegans) antibody
      • Lin 28 homolog A (C. elegans) antibody
      • Lin 28 homolog A antibody
      • Lin 28 homolog antibody
      • Lin-28A antibody
      • Lin28 antibody
      • Lin28, C. elegans, homolog of, A antibody
      • LIN28A antibody
      • LN28A_HUMAN antibody
      • Protein lin-28 homolog A antibody
      • Protein lin-28 homolog B antibody
      • RNA binding protein lin 28 antibody
      • Tex17 antibody
      • ZCCHC1 antibody
      • Zinc finger CCHC domain containing 1 antibody
      • Zinc finger CCHC domain containing protein 1 antibody
      • Zinc finger CCHC domain-containing protein 1 antibody
      see all

    Anti-Lin28 antibody [EPR4639] 图像

    • All lanes : Anti-Lin28 antibody [EPR4639] (ab109751) at 1/1000 dilution

      Lane 1 : JAR cell lysate
      Lane 2 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 23 kDa

    Anti-Lin28 antibody [EPR4639] (ab109751)参考文献

    ab109751 has not yet been referenced specifically in any publications.

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