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Our Abpromise guarantee covers the use of ab46020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/7000. PubMed: 20101213|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Ab46020 staining Lin28 in fetal ovary tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was fixed with Bouin’s fixative; heat mediated antigen retrieval was performed using 0.01M citrate and blocked with 20% serum for 1 hour at 20°C. Sample was incubated with primary antibody at 1/500 dilution for 16 hours at 4°C. A HRP conjugated goat anti-rabbit was used as the secondary antibody at 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"