概述

  • 产品名称Anti-LIMS1抗体[EP1944]
    参阅全部 LIMS1 一抗
  • 描述
    兔单克隆抗体[EP1944] to LIMS1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human LIMS1.

  • 阳性对照
    • 293T, HeLa, Jurkat, Human fetal kidney, and Human spleen cell lysates. Human kidney tissue. HeLa cells.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab108609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
    • 组织特异性Expressed in most tissues except in the brain.
    • 序列相似性Contains 5 LIM zinc-binding domains.
    • 细胞定位Cell junction > focal adhesion. Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2310016J22Rik antibody
      • 4921524A02Rik antibody
      • AI507642 antibody
      • AU021743 antibody
      • AW551584 antibody
      • C430041B13Rik antibody
      • LIM and senescent cell antigen like domains 1 antibody
      • LIM and senescent cell antigen-like-containing domain protein 1 antibody
      • LIM zinc finger domain containing 1 antibody
      • LIM-type zinc finger domains 1 antibody
      • Lims1 antibody
      • LIMS1_HUMAN antibody
      • Lims1l antibody
      • Particularly interesting new Cys His protein 1 antibody
      • Particularly interesting new Cys His protein antibody
      • Particularly interesting new Cys-His protein 1 antibody
      • PINCH 1 antibody
      • PINCH antibody
      • PINCH-1 antibody
      • PINCH1 antibody
      • Renal carcinoma antigen NY REN 48 antibody
      • Renal carcinoma antigen NY-REN-48 antibody
      • senescent cell antigen antibody
      • wu:fc32c03 antibody
      • zgc:112533 antibody
      see all

    Anti-LIMS1 antibody [EP1944] 图像

    • All lanes : Anti-LIMS1 antibody [EP1944] (ab108609) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Human fetal kidney lysate
      Lane 5 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108609 at 1/100 dilution.
    • Immunofluorescent staining of HeLa cells using ab108609 at 1/100 dilution.

    Anti-LIMS1 antibody [EP1944] (ab108609)参考文献

    This product has been referenced in:
    • Li A  et al. The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development. Proteomics 12:203-25 (2012). Read more (PubMed: 22253135) »

    See 1 Publication for this product

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