概述

  • 产品名称Anti-LIMS1抗体
    参阅全部 LIMS1 一抗
  • 描述
    兔多克隆抗体to LIMS1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chicken, Cow, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LIMS1.

    (Peptide available as ab88119.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysate: A549

性能

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应用

Our Abpromise guarantee covers the use of ab78814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
  • 组织特异性Expressed in most tissues except in the brain.
  • 序列相似性Contains 5 LIM zinc-binding domains.
  • 细胞定位Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt
  • 数据库链接
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Anti-LIMS1 antibody 图像

  • Anti-LIMS1 antibody (ab78814) at 1 µg/ml + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa


    Exposure time : 15 minutes
  • ICC/IF image of ab78814 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78814, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-LIMS1 antibody (ab78814)参考文献

ab78814 has not yet been referenced specifically in any publications.

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