概述

  • 产品名称
  • 描述
    兔多克隆抗体to LIME
  • 特异性
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    >95% by SDS-PAGE
  • 纯化说明
    Purified from rabbit antisera by precipitation methods.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 32.3 kDa.

靶标

  • 相关性
    LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
  • 细胞定位
    Cell membrane; Single-pass type III membrane protein. Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T cell with antigen presenting cell
  • 数据库链接
  • 别名
    • dJ583P15.4 antibody
    • FLJ20406 antibody
    • Lck interacting membrane protein antibody
    • Lck interacting molecule antibody
    • Lck interacting transmembrane adaptor 1 antibody
    • LIME 1 antibody
    • LIME1 antibody
    • LP8067 antibody
    • RP4 583P15.5 antibody
    see all

实验方案

文献

This product has been referenced in:
  • Chentouf M  et al. Recombinant anti-CD4 antibody 13B8.2 blocks membrane-proximal events by excluding the Zap70 molecule and downstream targets SLP-76, PLC gamma 1, and Vav-1 from the CD4-segregated Brij 98 detergent-resistant raft domains. J Immunol 179:409-20 (2007). WB ; Human . Read more (PubMed: 17579062) »
  • Lygren B  et al. A fast and sensitive method for isolation of detergent-resistant membranes from T cells. J Immunol Methods 305:199-205 (2005). Read more (PubMed: 16176818) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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