概述

  • 产品名称Anti-LIME抗体
    参阅全部 LIME 一抗
  • 描述
    兔多克隆抗体to LIME
  • 特异性Reacts with the cytoplasmic domain of LIME.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 141-295 of Human LIME bacterially expressed.

  • 阳性对照
    • Human Spleen tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab110094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
  • 细胞定位Cell membrane; Single-pass type III membrane protein. Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T cell with antigen presenting cell
  • 数据库链接
  • 别名
    • dJ583P15.4 antibody
    • FLJ20406 antibody
    • Lck interacting membrane protein antibody
    • Lck interacting molecule antibody
    • Lck interacting transmembrane adaptor 1 antibody
    • LIME 1 antibody
    • LIME1 antibody
    • LP8067 antibody
    • RP4 583P15.5 antibody
    see all

Anti-LIME antibody 图像

  • ab110094, at 10µg/ml, staining LIME in formalin-fixed, paraffin-embedded Human Spleen tissue by Immunohistochemistry.

Anti-LIME antibody (ab110094)参考文献

ab110094 has not yet been referenced specifically in any publications.

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