概述

  • 产品名称Anti-LIMD1抗体
    参阅全部 LIMD1 一抗
  • 描述
    小鼠单克隆抗体to LIMD1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment corresponding to amino acids 577-674 (VDSENKIYCV RDYHKVLAPK CAACGLPILP PEGSDETIRV VSMDRDYHVE CYHCEDCGLE LNDEDGHRCY PLEDHLFCHS CHVKRLEKRP SSTALHQH) of Human LIMD1 (NP_055055) with a proprietary tag.

  • 阳性对照
    • Human pancreas tissue, LIMD1 transfected 293T cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab118367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 72 kDa.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125oC for 4min and under 90oC for 45min.

靶标

  • 功能Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the zyxin/ajuba family.
    Contains 3 LIM zinc-binding domains.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AW822033 antibody
    • D9Ertd192e antibody
    • LIM domain-containing protein 1 antibody
    • LIM domains containing 1 antibody
    • LIMD1 antibody
    • LIMD1_HUMAN antibody
    • OTTHUMP00000164657 antibody
    • OTTHUMP00000210317 antibody
    • rCG_25194 antibody
    see all

Anti-LIMD1 antibody 图像

  • Anti-LIMD1 antibody (ab118367) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Predicted band size : 72 kDa
  • All lanes : Anti-LIMD1 antibody (ab118367) at 5 µg/ml

    Lane 1 : LIMD1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 72 kDa
  • Formalin-fixed paraffin-embedded Human pancreas tissue labelled with ab118367 at 3 µg/ml.

Anti-LIMD1 antibody (ab118367)参考文献

ab118367 has not yet been referenced specifically in any publications.

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