Anti-LIMD1抗体(ab114104)

概述

  • 产品名称Anti-LIMD1抗体
    参阅全部 LIMD1 一抗
  • 描述
    兔多克隆抗体to LIMD1
  • 经测试应用适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human LIMD1 (NP_055055.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab114104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-10 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the zyxin/ajuba family.
      Contains 3 LIM zinc-binding domains.
    • 细胞定位Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AW822033 antibody
      • D9Ertd192e antibody
      • LIM domain-containing protein 1 antibody
      • LIM domains containing 1 antibody
      • LIMD1 antibody
      • LIMD1_HUMAN antibody
      • OTTHUMP00000164657 antibody
      • OTTHUMP00000210317 antibody
      • rCG_25194 antibody
      see all

    Anti-LIMD1 antibody 图像

    • Detection of Human LIMD1 by Western blot of Immunoprecipitate.
      LIMD1 was immunoprecipitated from HeLa whole cell lysate, using ab114104 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-LIMD1 antibody was used for the subsequent WB at 0.4 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-LIMD1 antibody (ab114104)参考文献

    ab114104 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions Reduced Denaturing (10%)
    Loading amount 20 µg
    Specification HeLa
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Sep 22 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"