Anti-LIMD1抗体(ab114104)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to LIMD1
  • 经测试应用
    适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human LIMD1 (NP_055055.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab114104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-10 µg/mg of lysate.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    图片

    • Detection of Human LIMD1 by Western blot of Immunoprecipitate.
      LIMD1 was immunoprecipitated from HeLa whole cell lysate, using ab114104 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-LIMD1 antibody was used for the subsequent WB at 0.4 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    文献

    ab114104 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

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    Verified customer

    提交于 Sep 22 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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