概述

  • 产品名称
    Anti-LIMCH1抗体
  • 描述
    兔多克隆抗体to LIMCH1
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 734-1057 of Human LIMCH1.

  • 阳性对照
    • Mouse brain, H1299 and HeLaS3 whole cell lysates. IF/ICC: A549 cell line.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 119 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Suggest antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

靶标

  • 序列相似性
    Belongs to the LIMCH1 family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 LIM zinc-binding domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp434I0312 antibody
    • DKFZp686A01247 antibody
    • DKFZp686B2470 antibody
    • DKFZp686G18243 antibody
    • DKFZp686G2094 antibody
    • DKFZp781C1754 antibody
    • DKFZp781I1455 antibody
    • KIAA1102 antibody
    • LIM and calponin homology domains 1 antibody
    • LIM and calponin homology domains containing protein 1 antibody
    • LIM and calponin homology domains-containing protein 1 antibody
    • LIMC1_HUMAN antibody
    • LIMCH 1 antibody
    • limch1 antibody
    • LIMCH1A antibody
    • LMO7B antibody
    • MGC72127 antibody
    • OTTHUMP00000218619 antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab96178 antibody at 1:500 dilution.

  • Anti-LIMCH1 antibody (ab96178) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 119 kDa

    Run on 5% SDS PAGE gel

  • ICC/IF image of ab96178 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96178, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-LIMCH1 antibody (ab96178) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 119 kDa

文献

This product has been referenced in:
  • Bichell TJV  et al. Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model. Biochim Biophys Acta 1863:1596-1604 (2017). Read more (PubMed: 28213125) »

See 1 Publication for this product

客户评价及客户问答

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Application
Western blot
Sample
Human Tissue lysate - whole (Placenta)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Jeremiah Gaudet

Verified customer

提交于 Aug 03 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Specification
Placenta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Jeremiah Gaudet

Verified customer

提交于 Jul 27 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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