Anti-LIMCH1抗体(ab96178)

概述

  • 产品名称Anti-LIMCH1抗体
    参阅全部 LIMCH1 一抗
  • 描述
    兔多克隆抗体to LIMCH1
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 734-1057 of Human LIMCH1.

  • 阳性对照
    • Mouse brain, H1299 and HeLaS3 whole cell lysates. IF/ICC: A549 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 119 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Suggest antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

靶标

  • 序列相似性Belongs to the LIMCH1 family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 LIM zinc-binding domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp434I0312 antibody
    • DKFZp686A01247 antibody
    • DKFZp686B2470 antibody
    • DKFZp686G18243 antibody
    • DKFZp686G2094 antibody
    • DKFZp781C1754 antibody
    • DKFZp781I1455 antibody
    • KIAA1102 antibody
    • LIM and calponin homology domains 1 antibody
    • LIM and calponin homology domains containing protein 1 antibody
    • LIM and calponin homology domains-containing protein 1 antibody
    • LIMC1_HUMAN antibody
    • LIMCH 1 antibody
    • limch1 antibody
    • LIMCH1A antibody
    • LMO7B antibody
    • MGC72127 antibody
    • OTTHUMP00000218619 antibody
    see all

Anti-LIMCH1 antibody 图像

  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab96178 antibody at 1:500 dilution.

  • Anti-LIMCH1 antibody (ab96178) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 119 kDa

    Run on 5% SDS PAGE gel

  • ICC/IF image of ab96178 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96178, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-LIMCH1 antibody (ab96178) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 119 kDa

Anti-LIMCH1 antibody (ab96178)参考文献

ab96178 has not yet been referenced specifically in any publications.

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I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any LIMCH1 antibody tested in the s...

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