概述

  • 产品名称Anti-LIM1抗体
    参阅全部 LIM1 一抗
  • 描述
    兔多克隆抗体to LIM1
  • 特异性Does not appear to crossreact with LIM5 (the closest homologue of LIM1 in frog) on tissue sections but does crossreact with LIM5 in Western blot and immunoprecipitation.
  • 经测试应用适用于: IHC-P, ICC, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Xenopus laevis, Fish
  • 免疫原

    C-terminal portion of frog LIM-1 protein.

  • 阳性对照
    • Tested with P19 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab14554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/1000.
ICC 1/500.
WB 1/3000 - 1/6000. Predicted molecular weight: 44 kDa.
IP 1/200.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
  • 组织特异性Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
  • 序列相似性Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • 结构域The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hLim-1 antibody
    • Homeo box protein Lim 1 antibody
    • Homeo box protein Lim1 antibody
    • Homeobox protein Lim 1 antibody
    • Homeobox protein Lim-1 antibody
    • Homeobox protein Lim1 antibody
    • LHX 1 antibody
    • LHX1 antibody
    • LHX1_HUMAN antibody
    • LIM 1 antibody
    • LIM homeo box 1 antibody
    • LIM homeo box protein 1 antibody
    • LIM homeobox 1 antibody
    • LIM homeobox protein 1 antibody
    • LIM-1 antibody
    • LIM/homeobox protein Lhx 1 antibody
    • LIM/homeobox protein Lhx1 antibody
    • MGC126723 antibody
    • MGC138141 antibody
    see all

Anti-LIM1 antibody 图像

  • ab14554 staining LIM1 in human fetal kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Actin filaments stained with phalloidin (red).

    See Abreview

  • ab14554 at 1/200 dilution staining mouse postnatal day 5 retina cells (known to express LIM 1) by ICC/IF. The cells were formaldehyde fixed and incubated with the antibody for 72 hours. An Alex-Fluor 488 conjugated donkey anti-rabbit antibody was used as the secondary. The image shows strong horizontal cell process labeling with fainter nuclear label.

    See Abreview

Anti-LIM1 antibody (ab14554)参考文献

This product has been referenced in:
  • Di Giovannantonio LG  et al. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development 141:377-88 (2014). Read more (PubMed: 24335253) »
  • Amirpour N  et al. Differentiation of Human Embryonic Stem Cell-Derived Retinal Progenitors into Retinal Cells by Sonic Hedgehog and/or Retinal Pigmented Epithelium and Transplantation into the Subretinal Space of Sodium Iodate-Injected Rabbits. Stem Cells Dev : (2011). IHC-Fr ; Rabbit . Read more (PubMed: 21456900) »

See all 3 Publications for this product

Product Wall

Sure, as the antibody is not working as expected, I would be happy to send a replacement or offer a credit or refund. What would you prefer?

Application Immunohistochemistry (Frozen sections)
Sample Chicken Tissue sections (nephric duct (chiken))
Permeabilization Yes - 0.25% triton in PBS
Specification nephric duct (chiken)
Blocking step (agent) for 15 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative Paraformaldehyde
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提交于 Nov 24 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (fetal kidney cells)
Specification fetal kidney cells
Permeabilization Yes - 0.5% triton in pbs
Fixative Formaldehyde
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提交于 Oct 24 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Chicken Tissue sections (E5 Spinal cord)
Specification E5 Spinal cord
Permeabilization Yes - triton x-100
Fixative Paraformaldehyde
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Verified customer

提交于 Nov 27 2013

Thank you for your reply. I am sending a vial of ab82844 free of charge on the order ***. This is in stock and I expect it to arrive tomorrow. Please keep me updated on the results with this replacement antibody, and let me know if you h...

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Thanks for your reply and I look forward to receiving your image. Regarding the 150 kDa band, I have looked through our records and briefly in the literature and have found no evidence that LIM1 should run that high, so this band is most likely ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (postnatal day 5 retina)
Specification postnatal day 5 retina
Fixative Formaldehyde
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
Username

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提交于 Oct 17 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"