Synthetic phosphopeptide derived from Human LIM kinase 2 around the phosphorylation site of serine 283 (R-R-SP-L-R).
Human brain tissue
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液pH: 7.40 Preservative: 0.02% Sodium azide Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride Note: PBS is without Mg2+ and Ca2+.
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纯度Immunogen affinity purified
纯化说明ab111589 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
组织特异性Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 2 LIM zinc-binding domains. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain.
翻译后修饰Phosphorylated on serine and/or threonine residues by ROCK1.
细胞定位Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.