Anti-LIM kinase 2 (phospho S283)抗体(ab111589)

概述

  • 产品名称Anti-LIM kinase 2 (phospho S283)抗体
    参阅全部 LIM kinase 2 一抗
  • 描述
    兔多克隆抗体to LIM kinase 2 (phospho S283)
  • 特异性ab111589 detects endogenous levels of LIM kinase 2 only when phosphorylated at serine283.
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human LIM kinase 2 around the phosphorylation site of serine 283 (R-R-SP-L-R).

  • 阳性对照
    • Human brain tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab111589 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • 组织特异性Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated on serine and/or threonine residues by ROCK1.
  • 细胞定位Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Anti-LIM kinase 2 (phospho S283) antibody 图像

  • ab111589, at a 1/50 dilution, staining LIM kinase in paraffin embedded Human brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-LIM kinase 2 (phospho S283) antibody (ab111589)参考文献

ab111589 has not yet been referenced specifically in any publications.

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