概述
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产品名称Anti-LIM kinase 2抗体
参阅全部 LIM kinase 2 一抗 -
描述兔多克隆抗体to LIM kinase 2
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Recombinant fragment, corresponding to amino acids 1 - 252 of Human LIM kinase 2
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阳性对照
- 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji cell lines; A549 cells
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab97773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 72 kDa. | |
| ICC/IF | 1/100 - 1/200. |
靶标
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功能Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
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组织特异性Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
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序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
翻译后修饰Phosphorylated on serine and/or threonine residues by ROCK1.
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细胞定位Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 513539 Cow
- Entrez Gene: 3985 Human
- Entrez Gene: 16886 Mouse
- Entrez Gene: 29524 Rat
- Omim: 601988 Human
- SwissProt: Q32L23 Cow
- SwissProt: P53671 Human
- SwissProt: O54785 Mouse
see all -
别名
- LIM domain kinase 2 antibody
- LIMK 2 antibody
- LIMK-2 antibody
see all
Anti-LIM kinase 2 antibody 图像
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Western blot - LIM kinase 2 antibody (ab97773)All lanes : Anti-LIM kinase 2 antibody (ab97773) at 1/2000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 72 kDa -
Immunocytochemistry/ Immunofluorescence - LIM kinase 2 antibody (ab97773)ab97773, at a 1/200 dilution, staining LIM kinase 2 in paraformaldehyde fixed A549 cells by Immunofluorescence analysis. The lower image (panel 2) was merged with DNA probe.
Anti-LIM kinase 2 antibody (ab97773)参考文献
ab97773 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"