Anti-LIM kinase 2抗体(ab97773)

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概述

  • 产品名称
    Anti-LIM kinase 2抗体
    参阅全部 LIM kinase 2 一抗
  • 描述
    兔多克隆抗体to LIM kinase 2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1 - 252 of Human LIM kinase 2

  • 阳性对照
    • 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji cell lines; A549 cells

性能

应用

Our Abpromise guarantee covers the use of ab97773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • 组织特异性
    Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • 序列相似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylated on serine and/or threonine residues by ROCK1.
  • 细胞定位
    Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

图片

  • Western blot - Anti-LIM kinase 2 antibody (ab97773)
    Western blot - Anti-LIM kinase 2 antibody (ab97773)
    All lanes : Anti-LIM kinase 2 antibody (ab97773) at 1/2000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 72 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-LIM kinase 2 antibody (ab97773)
    Immunocytochemistry/ Immunofluorescence - Anti-LIM kinase 2 antibody (ab97773)
    ab97773, at a 1/200 dilution, staining LIM kinase 2 in paraformaldehyde fixed A549 cells by Immunofluorescence analysis. The lower image (panel 2) was merged with DNA probe.

文献

ab97773 has not yet been referenced specifically in any publications.

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